LOCUS       sequence-162473-        4218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM45, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM45_WT
FEATURES             Location/Qualifiers
     source          1..4218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1618..1717)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1734..1753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1735..1753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1758..1774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1758..1774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2205..2996
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3089..3677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3578..3597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3831..3848
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4007..4034)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4126..4212)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggacgaa gctggcagct ctgcgagcgg cgggggcttc cgcccgggcg
      241 tggacagcct ggacgaaccg cccaacagcc gcatcttcct tgtgatcagc aagtacacac
      301 ctgagtcggt gctgagggag cgcttctcgc cttttggcga catccaggac atctgggtgg
      361 tgcgggacaa gcacaccaag gagtccaagg gcattgcttt cgtcaagttc gcccgcagct
      421 cacaggcctg cagggccatg gaggagatgc atggccagtg cctcggcccc aacgacacca
      481 agcccatcaa ggttttcatt gctcagtccc gatcatctgg aagtcaccga gatgttgaag
      541 atgaagaact tacaagaatc tttgttatga taccaaagtc ctacacagaa gaagatctgc
      601 gggaaaaatt taaggtgtat ggagatatcg agtattgcag cattattaag aataaagtga
      661 ctggagaaag taaaggtttg ggctacgtac gatacttaaa accatcacaa gctgcccaag
      721 caatagaaaa ctgtgatcga agttttagag caatcttggc tgaacctaaa aataaagcat
      781 ctgaatcctc tgaacaagat tattatagta atatgaggca agaagctttg ggacatgaac
      841 ctagagtaaa tatgtttcca tttgaacaac aatctgaatt ttcaagtttt gacaagaatg
      901 atagccgagg ccaggaagca atctccaaac gcttgtcagt tgtatcaaga gttcctttca
      961 ctgaagaaca gcttttcagc atttttgata tagtaccagg attggaatat tgtgaagttc
     1021 aacgagatcc ttattcaaat tatggtcatg gagtggttca gtattttaat gtagcatcag
     1081 ctatttatgc aaaatacaaa ttacatggat ttcagtaccc tcctgggaac cgaataggtg
     1141 tttccttcat tgatgatgga agtaatgcaa cagatctcct tagaaaaatg gcaacacaga
     1201 tggtagctgc acagcttgca tcaatggtgt ggaataaccc aagtcagcaa caatttatgc
     1261 aatttggagg aagctctgga tcacagttgc ctcaaatcca gacagatgtt gtacttccat
     1321 catgcaaaaa aaaagctcct gctgaaactc ctgtgaaaga aagacttttt attgtgttta
     1381 atcctcatcc tttaccttta gacgtattag aagatatatt ctgtcgtttt ggtaacctga
     1441 tcgaagttta ccttgtgtca ggaaaaaatg tggggtatgc caagtatgcc gatagaataa
     1501 gtgctaatga tgccattgcc actctacatg gaaagattct gaatggggtg agacttaaag
     1561 ttatgctggc agattcgcca agagaagaat ctaacaaacg gcaaagaact tactgattgc
     1621 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1681 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1741 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1801 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     1861 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     1921 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     1981 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2041 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2101 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2161 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2221 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2281 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2341 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2401 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2461 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2521 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2581 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2641 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2701 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2761 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2821 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2881 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2941 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3001 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3841 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     3901 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     3961 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     4021 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4081 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4141 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4201 tcgttttatt tgatgcct
//
