LOCUS       sequence-162495-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of GFP tagged mutant BuGZ in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUT BuGZ delta ZF1, delta ZF2
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..738
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
ORIGIN
        1 gcgcgccagt cctccgacag actgagtcgc ccgctggaga ccatggagag cgacgagagc
       61 ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc
      121 gagctggtgg gcggcggaga gggcaccccc gagcagggcc gcatgaccaa caagatgaag
      181 agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc
      241 ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac
      301 aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg
      361 agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt gatgggcacc
      421 ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg
      481 gagcacctgc accccatggg cgataacgat ctggatggca gcttcacccg caccttcagc
      541 ctgcgcgacg gcggctacta cagctccgtg gtggacagcc acatgcactt caagagcgcc
      601 atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag
      661 gatcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa gaccccggat
      721 gcagatgccg gtgaagaata catgggtcgc aagaagaaga agcagctgaa gccgtggaa
//
