LOCUS       sequence-162543-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Late2(delta)-4GS-I3-01-myc-PH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN20 (deltaL)
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             809..838
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acgggccctc tagaattatc gatccggagg
      121 taccatgcgc cgcgtgatcc tggcagctgc ggcagctgaa tatatggaag cgatttaccc
      181 agttcgcggt agcggtagca agatcgaaga gctgttcaag aaacacaaga tcgttgccgt
      241 gctgcgtgcc aatagtgtgg aagaagcgaa aaagaaagcg ctggcggttt tcctgggcgg
      301 cgttcatctg attgaaatta cctttaccgt gccggatgcg gataccgtga ttaaggaact
      361 gagctttctg aaggaaatgg gcgcgattat tggtgcgggc accgtgacca gcgtggagca
      421 gtgccgtaaa gcggtggaaa gtggcgccga attcattgtg agtccgcacc tggacgagga
      481 aattagccaa ttttgcaagg agaagggtgt gttctatatg ccaggcgtta tgaccccgac
      541 cgaactggtg aaagccatga aactgggcca taccatctta aaactgtttc cgggtgaggt
      601 ggtgggtccg cagtttgtta aagcgatgaa aggtccgttt ccgaatgtga aatttgtgcc
      661 aaccggcggt gttaatctgg acaatgtgtg cgaatggttc aaagcgggcg tgctggccgt
      721 gggcgtgggc agcgcgttag tgaaaggcac cccggtggaa gtggcggaaa aggccaaggc
      781 gttcgttgag aagattcgtg gctgcaccga acagaagctg atctccgaag aagacctgca
      841 cggtctgcag gacgacccgg acctgcaagc actgctcaag ggcagccagc tgctgaaagt
      901 taaatcttct tcttggcgtc gtgaacgttt ctacaaactc caggaggact gcaaaaccat
      961 ctggcaggaa tcccgtaaag ttatgcgttc tccggaatct cagctgttct ctatc
//
