LOCUS       sequence-162591-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myr2-12GS-I3-01-myc-Late1 (1wa3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Votteler J, Ogohara C, Yi S, Hsia Y, Nattermann U, Belnap DM, King 
            NP, Sundquist WI
  TITLE     Designed proteins induce the formation of nanocage-containing 
            extracellular vesicles.
  JOURNAL   Nature. 2016 Dec 8;540(7632):292-295. doi: 10.1038/nature20607. Epub
            2016 Nov 30.
  PUBMED    27919066
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature20607"; journalName: "Nature"; date: "2016-12-8- 8"; 
            volume: "540"; issue: "7632"; pages: "292-295"
COMMENT     Sequence Label: pCMV-EPN-03 (1wa3)
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             787..816
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cgggccctct agaattatcg atccggaggt
      121 accatgggtg cacagttttc cggttctaaa tctggctccg gctctgactc tggtagcaag
      181 atcgaagagc tgttcaagaa acacaagatc gttgccgtgc tgcgtgccaa tagtgtggaa
      241 gaagcgaaag aaaaagcgct ggcggttttc gaaggcggcg ttcatctgat tgaaattacc
      301 tttaccgtgc cggatgcgga taccgtgatt aaggaactga gctttctgaa ggaaaaaggc
      361 gcgattattg gtgcgggcac cgtgaccagc gtggagcagt gccgtaaagc ggtggaaagt
      421 ggcgccgaat tcattgtgag tccgcacctg gacgaggaaa ttagccaatt ttgcaaggag
      481 aagggtgtgt tctatatgcc aggcgttatg accccgaccg aactggtgaa agccatgaaa
      541 ctgggccata ccatcttaaa actgtttccg ggtgaggtgg tgggtccgca gtttgttaaa
      601 gcgatgaaag gtccgtttcc gaatgtgaaa tttgtgccaa ccggcggtgt taatctggac
      661 aatgtgtgcg aatggttcaa agcgggcgtg ctggccgtgg gcgtgggcag cgcgttagtg
      721 aaaggcaccc cggatgaagt gcgtgaaaag gccaaggcgt tcgttgagaa gattcgtggc
      781 tgcaccgaac agaaactgat tagcgaagaa gatctgcagt ctcgtccgga accgaccgca
      841 ccgccggaag aatctttccg ttctggtgtt gaaaccacca cgccaccgca aaaacaggag
      901 ccgatcgaca aagaactgta cccgctgacc tctctgcgtt ctctgttcgg taacgacccg
      961 tcttctcagt gactcgagaa gcttgctagc agatctt
//
