LOCUS       sequence-162625-       12741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for N-terminal fusion with 
            eCFP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12741)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 12741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB545
FEATURES             Location/Qualifiers
     source          1..12741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1100..1120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1178..1180,1181..1183,1184..1894)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1223..1244)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1484..1503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1831..1852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1904..2028
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2081..2086,2087..2104,2105..2111)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2165..2824
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2231..2250)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3166..3471
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3350..3369
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3512..3636)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3662..3914
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3948..3964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3948..3964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3961..3983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3972..3988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3996..4002,4003..4020,4021..4026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4041..4062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4098..4350)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4501..5526)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5578..5757)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6263..6287
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6808..7599
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7845..8433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8334..8353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8587..8604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8619..8759
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8685..8704)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8845..8867
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9103..9297
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9363..10436)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10865..11494)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct
     1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
     1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag
     1621 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1861 cgggatcact ctcggcatgg acgagctgta caaggctgtt atcacaagtt tgtacaaaaa
     1921 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1981 aaaacagact acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga
     2041 ggcctcgagg cctcgagggg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2101 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     2161 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2221 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     2281 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2341 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     2401 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2461 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2521 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2581 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2641 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2701 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt
     2761 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     2821 gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     2881 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt
     2941 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     3001 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     3061 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     3121 ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt
     3181 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     3241 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     3301 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     3361 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     3421 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     3481 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     3541 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     3601 ttacgtttct cgttcagctt tcttgtacaa agtggtgata acctagagct cgaatttccc
     3661 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     3721 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     3781 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     3841 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     3901 tatgttacta gatcgggaat tggttccgga accaattcgt aatcatggtc atagctgttt
     3961 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4021 tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc
     4081 tatttctatt aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta
     4141 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
     4201 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
     4261 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
     4321 gaaactttat tgccaaatgt ttgaacgatc ggggaaattc gggggatcga tcccgcgaac
     4381 tgtggacgag aactgtgcca ccaagcgtaa ggccgttctc tcgcatttgc cttgctaggc
     4441 tcgcgcgagt tgctggctga ggcgttctcg aaatcagctc ttgttcggtc ggcatctact
     4501 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     4561 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     4621 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     4681 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     4741 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     4801 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     4861 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     4921 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     4981 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     5041 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     5101 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     5161 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     5221 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     5281 tgccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     5341 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     5401 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     5461 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     5521 tttcatatct tattgccccc cgggatcaga tctggattga gagtgaatat gagactctaa
     5581 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     5641 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     5701 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt
     5761 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc
     5821 ttaattctcc gctcatgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     5881 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     5941 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     6001 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     6061 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta
     6121 gcagcccttg cgccctgagt gcttgcggca gcgtgaagct gatccgtcga gattaataga
     6181 aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     6241 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     6301 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     6361 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     6421 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     6481 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     6541 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     6601 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     6661 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     6721 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     6781 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     6841 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     6901 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     6961 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     7021 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     7081 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     7141 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     7201 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     7261 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     7321 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     7381 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     7441 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     7501 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     7561 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     7621 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     7681 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
     7741 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg
     7801 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7861 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7921 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7981 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8041 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8101 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8161 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8221 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8281 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8461 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8521 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8581 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     8641 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     8701 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     8761 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8821 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     8881 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     8941 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     9001 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     9061 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     9121 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     9181 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     9241 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     9301 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     9361 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     9421 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     9481 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     9541 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     9601 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     9661 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     9721 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     9781 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     9841 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     9901 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     9961 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
    10021 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
    10081 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
    10141 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
    10201 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
    10261 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
    10321 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
    10381 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
    10441 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
    10501 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
    10561 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
    10621 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
    10681 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
    10741 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
    10801 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
    10861 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
    10921 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
    10981 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
    11041 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
    11101 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    11161 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    11221 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    11281 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    11341 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    11401 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    11461 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    11521 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    11581 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    11641 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    11701 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    11761 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    11821 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    11881 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    11941 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    12001 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    12061 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    12121 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    12181 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    12241 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    12301 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    12361 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    12421 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    12481 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    12541 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    12601 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    12661 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    12721 aatggacgaa cggataaacc t
//
