LOCUS       sequence-162724-        4212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing RBM45, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_RBM45_WT_V5
FEATURES             Location/Qualifiers
     source          1..4212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     419..436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(595..622)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(714..800)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     849..871
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     863..880
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     864..880
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    896..995
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2418..2517)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2534..2553)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2535..2553)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2558..2574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2558..2574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3005..3796
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3889..4212,1..265)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
       61 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
      421 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
      481 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
      541 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
      601 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
      661 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
      721 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
      781 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
      841 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat
      901 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
      961 ttttataatg ccaactttgt acaaaaaagt tggcatggac gaagctggca gctctgcgag
     1021 cggcgggggc ttccgcccgg gcgtggacag cctggacgaa ccgcccaaca gccgcatctt
     1081 ccttgtgatc agcaagtaca cacctgagtc ggtgctgagg gagcgcttct cgccttttgg
     1141 cgacatccag gacatctggg tggtgcggga caagcacacc aaggagtcca agggcattgc
     1201 tttcgtcaag ttcgcccgca gctcacaggc ctgcagggcc atggaggaga tgcatggcca
     1261 gtgcctcggc cccaacgaca ccaagcccat caaggttttc attgctcagt cccgatcatc
     1321 tggaagtcac cgagatgttg aagatgaaga acttacaaga atctttgtta tgataccaaa
     1381 gtcctacaca gaagaagatc tgcgggaaaa atttaaggtg tatggagata tcgagtattg
     1441 cagcattatt aagaataaag tgactggaga aagtaaaggt ttgggctacg tacgatactt
     1501 aaaaccatca caagctgccc aagcaataga aaactgtgat cgaagtttta gagcaatctt
     1561 ggctgaacct aaaaataaag catctgaatc ctctgaacaa gattattata gtaatatgag
     1621 gcaagaagct ttgggacatg aacctagagt aaatatgttt ccatttgaac aacaatctga
     1681 attttcaagt tttgacaaga atgatagccg aggccaggaa gcaatctcca aacgcttgtc
     1741 agttgtatca agagttcctt tcactgaaga acagcttttc agcatttttg atatagtacc
     1801 aggattggaa tattgtgaag ttcaacgaga tccttattca aattatggtc atggagtggt
     1861 tcagtatttt aatgtagcat cagctattta tgcaaaatac aaattacatg gatttcagta
     1921 ccctcctggg aaccgaatag gtgtttcctt cattgatgat ggaagtaatg caacagatct
     1981 ccttagaaaa atggcaacac agatggtagc tgcacagctt gcatcaatgg tgtggaataa
     2041 cccaagtcag caacaattta tgcaatttgg aggaagctct ggatcacagt tgcctcaaat
     2101 ccagacagat gttgtacttc catcatgcaa aaaaaaagct cctgctgaaa ctcctgtgaa
     2161 agaaagactt tttattgtgt ttaatcctca tcctttacct ttagacgtat tagaagatat
     2221 attctgtcgt tttggtaacc tgatcgaagt ttaccttgtg tcaggaaaaa atgtggggta
     2281 tgccaagtat gccgatagaa taagtgctaa tgatgccatt gccactctac atggaaagat
     2341 tctgaatggg gtgagactta aagttatgct ggcagattcg ccaagagaag aatctaacaa
     2401 acggcaaaga acttactgcc caactttctt gtacaaagtt ggcattataa gaaagcattg
     2461 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     2521 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     2581 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     2641 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     2701 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     2761 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     2821 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     2881 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     2941 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     3001 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     3061 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     3121 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     3181 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3241 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3301 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3361 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3421 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3481 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3541 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3601 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3661 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3721 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     3781 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     3841 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3901 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3961 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4021 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4081 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4141 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4201 gggctgaacg gg
//
