LOCUS       sequence-162727-        4293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PWP1, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_PWP1_WT_V5
FEATURES             Location/Qualifiers
     source          1..4293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(689..788)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(804..821)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(804..820)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(813..835)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      884..970
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1062..1089
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1248..1265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1419..2007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1499..1518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2100..2891)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     3322..3338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3322..3338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3343..3362
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3343..3361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3379..3478
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ggctctaaag agtccactat atcaaggtcc cacacttcaa taacaggggt catgtttcct
       61 acagcaatgt aatttccagt agaatcatct gggctaggat caaaattcag ccattccaca
      121 ctcagaggat atgcagacaa gagtatatca tggtgtacat aaaaagagtc ttcttcttga
      181 ttataaacat gcacgtctaa attgcactgg tcctgttcag ctcggccaca aactataaga
      241 ttatcactgg gcttaatcaa gaaatcttca cgttcatatt gttctgtatc tttcagagta
      301 acgtaaggat cttgatcatt actcccgtag accgtaagac ccaagagaga ttcaccaaga
      361 gtctcagcat ctgggtcacc ttcctcatca tatttatcta agtcgtactc agccagctca
      421 tcatcatcaa gcgtcctgtc atcctctggg tcaccatcct ccaggggctc tcttgggcgt
      481 gcctgggtgc gtgcactctg catgccatct tctgaaggac tgcctgtctc ctcttcatca
      541 ctgccaccac cttcttcttg caatttctcc tttgcctcag caatgaggcg ttttacttct
      601 tctttactca gctctacctt gtctggtgtc tctttggcca cgccgcagcg gacccaggcc
      661 acgcacgtca cctggcggct gcggttcatg ccaacttttt tgtacaaagt tggcattata
      721 aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
      781 attatttggg gcccgagctt aagactggcc gtcgttttac aacgtcgtga ctgggaaaac
      841 atccatgcta gcgttaacgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
      901 tcagtcggaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
      961 taggacaaat ccgccgggag cggatttgaa cgttgtgaag caacggcccg gagggtggcg
     1021 ggcaggacgc ccgccataaa ctgccaggca tcaaactaag cagaaggcca tcctgacgga
     1081 tggccttttt gcgtttctac aaactcttcc tggctagcgg tacgcgtatt aattgcgttg
     1141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2041 gctcagtgga acgacgcgta actcacgtta agggattttg gtcatgggtg gctcgagggt
     2101 tatttgccga ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga
     2161 tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt
     2221 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     2281 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     2341 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     2401 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     2461 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     2521 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     2581 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     2641 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     2701 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     2761 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     2821 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     2881 gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     2941 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     3001 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
     3061 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
     3121 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
     3181 cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg
     3241 ctggtctaga ggaggcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     3301 tgagacacgg gccagagctg ccaggaaaca gctatgacca tgtaatacga ctcactatag
     3361 gggatatcag ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg
     3421 caacaaattg ataagcaatg ctttcttata atgccaactt tgtacaagaa agttgggtaa
     3481 gactccatgg gtgtatctga gctcctgctg ccaaaagggc cactaataga tgaatttctt
     3541 gcactcccaa gaacaagcct ctctcgtctt ccaaatgctt catttactga agagactgtg
     3601 cttatatccc agacccgaag cccttctttt tgacctcgaa gggcataaat aaatggcaaa
     3661 tcagggcaac atgaagaaca gaagagaact cccattttca tgtccctaga atgaactaga
     3721 cttggcctat ctcctaagat gtcccagatc ttcacgtatt tgtcagctga agcagtcacg
     3781 agacagccct tgatttgact gctaagatca agaccagaga tttcatcatt gtgtgcatta
     3841 agtgtaaaaa ttggcttatc tgaacgtgca tccaaattat atacaaagcc gtcatctgta
     3901 ctggccaaga aatgacaagg tgaaaagtga ttccaagtca ctctctctat ctgcccactg
     3961 aatcgccaca ttcgatggct ttcatctgga cttcggcagt catacaaagc cactgactta
     4021 tcatatgagc cagaaatcag agtctgtgct tcaaatggat gaaactgcag tgtttggacc
     4081 ttgtctgtgt gtacagcgag gctagctgct ggtttcccca aggacatatc ccacagaatt
     4141 acagtgttgt cagctgatgc acttgctaaa acatttctga ttagcttatt ccatgaaagg
     4201 tcaaggacag catcggtatg cccttctgct gaggaactct tctttccttt ctttttcttc
     4261 ttttttgaaa gtttacttcc gagtgtgaag act
//
