LOCUS       sequence-162765-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cre-dependent DN-AMPK under EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kong D, Dagon Y, Campbell JN, Guo Y, Yang Z, Yi X, Aryal P, 
            Wellenstein K, Kahn BB, Sabatini BL, Lowell BB
  TITLE     A Postsynaptic AMPK-->p21-Activated Kinase Pathway Drives 
            Fasting-Induced Synaptic Plasticity in AgRP Neurons.
  JOURNAL   Neuron. 2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025.
  PUBMED    27321921
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jun 16. pii: S0896-6273(16)30204-5. doi: 
            10.1016/j.neuron.2016.05.025."
COMMENT     Sequence Label: pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..73)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(124..157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             170..199
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat
       61 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg
      121 caaataactt cgtataggat actttatacg aagttatgct agcgccatgg agcagaagct
      181 tatctccgag gaggacctcg gtggcggcga gaagcagaag cacgacgggc gtgtgaagat
      241 cggacactac gtgctggggg acaccctggg cgtcggcacc ttcggcaaag tgaagattgg
      301 agaacatcaa ttgacaggcc ataaagtggc agttaggatc ttaaatagac agaagattcg
      361 cagtttagat gttgttggaa aaataaaacg agaaattcaa aatcttaaac tctttcgtca
      421 tcctcatatt atcaaactct accaagtgat cagcactcca acagactttt ttatggtaat
      481 ggaatatgtg tctggaggtg aattgttcga ctacatctgt aaacacggga gggttgaaga
      541 ggtggaagct cgccggctct tccagcagat tctgtctgcc gtggactact gtcacaggca
      601 catggttgtc cacagggacc tgaagccaga gaacgtgttg ctggacgccc agatgaatgc
      661 taagatagct gacttcggac tctctaatat gatgtcagat ggtgaatttc tacgaactag
      721 ctgtggatcg ccaaattatg cagcaccgga ggtcatctca ggaaggctgt atgcgggtcc
      781 tgaggttgat atctggagct gtggtgttat cctgtatgcc cttctctgtg gcacc
//
