LOCUS       sequence-162786-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for 
            monitoring autophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Tanida I, Ueno T, Uchiyama Y
  TITLE     A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged
            Human LC3 for the Monitoring of Mammalian Autophagy.
  JOURNAL   PLoS One. 2014 Oct 23;9(10):e110600. doi: 
            10.1371/journal.pone.0110600. eCollection 2014.
  PUBMED    25340751
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-23"; volume: "9(10):e110600. doi"; pages: " 
            10.1371/journal.pone.0110600. eCollection 2014"
COMMENT     Sequence Label: pEX-PK-hLC3
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             422..796
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
ORIGIN
        1 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
       61 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
      121 ggaggcctcc accgagaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat
      181 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc
      241 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga
      301 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
      361 atactgcgac ctccctagca aactggggca cagaggtgga ggaggttccg gactcagatc
      421 tatgccgtcg gagaagacct tcaagcagcg ccgcaccttc gaacaaagag tagaagatgt
      481 ccgacttatt cgagagcagc atccaaccaa aatcccggtg ataatagaac gatacaaggg
      541 tgagaagcag cttcctgttc tggataaaac aaagttcctt gtacctgacc atgtcaacat
      601 gagtgagctc atcaagataa ttagaaggcg cttacagctc aatgctaatc aggccttctt
      661 cctgttggtg aacggacaca gcatggtcag cgtctccaca ccaatctcag aggtgtatga
      721 gagtgagaaa gatgaagatg gattcctgta catggtctac gcctcccagg agacgttcgg
      781 gatgaaattg tcagtgtaag tcgagcccgg gagctcaatt cactcctcag gtgcaggctg
      841 cctatcagaa ggtggtggct
//
