LOCUS       sequence-162833-         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and 
            miR-19 binding sites. To be used for 3'UTR assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Murphy BL, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, 
            Roussel MF
  TITLE     Silencing of the miR-17~92 cluster family inhibits medulloblastoma 
            progression.
  JOURNAL   Cancer Res. 2013 Oct 21.
  PUBMED    24145352
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2013 Oct 21."
COMMENT     Sequence Label: pmirGLO-Smad4-1719
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(217..235)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    272..393
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(283..302)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     337..356
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        638..829
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(661..681)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca
       61 agaagggcgg caagatcgcc gtgtaattct agttgtttaa actagcggcc gctagttttt
      121 tttcttttgc acttttgatc tagagtcgac ctgcaggcat gcaagctgat ccggctgcta
      181 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      241 cccttggggc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
      301 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      361 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      421 tttcaggttc agggggagat gtgggaggtt tttttaagca agtaaaacct ctacaaatgt
      481 ggtaaaatcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
      541 cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca ccatggcctg
      601 aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag aaccagctgt
      661 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      721 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
      781 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
      841 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
      901 ttttttt
//
