LOCUS       sequence-162860-        5387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 558, Emission:
            583.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DsRed2-pBAD
FEATURES             Location/Qualifiers
     source          1..5387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..145
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(71..90)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     231..253
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     391..410
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(590..1468)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1495..1779
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1702..1721
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1806..1828
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1848..1865
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1869..1901
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1914..1934
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1941..2618
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2121..2141)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2533..2556
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2664..2681)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2834..2920
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3012..3039
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3058..3149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3150..3218,3219..4010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3368..3387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4052..4507
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4139..4158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4349..4370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      4618..5206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5107..5126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5360..5377
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatctggt
       61 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
      121 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      181 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      241 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagaagg agatggcgcc
      301 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
      361 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
      421 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctgctcatg
      481 tttgacagct tatcatcgat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
      541 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
      601 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
      661 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
      721 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
      781 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
      841 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
      901 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
      961 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
     1021 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
     1081 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
     1141 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
     1201 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
     1261 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
     1321 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
     1381 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
     1441 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
     1501 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     1561 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     1621 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     1681 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatacta
     1741 cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagaa
     1801 ataattttgt ttaactttaa gaaggagata tacatatgcg gggttctcat catcatcatc
     1861 atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg tacgagaacc
     1921 tgtacttcca gggctcgagc atggcctcct ccgagaacgt catcaccgag ttcatgcgct
     1981 tcaaggtgcg catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     2041 agggccgccc ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc
     2101 tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga
     2161 agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg
     2221 agcgcgtgat gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc
     2281 aggacggctg cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc
     2341 ccgtgatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
     2401 acggcgtgct gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc
     2461 tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact
     2521 acgtggacgc caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt
     2581 acgagcgcac cgagggccgc caccacctgt tcctgtaaga attcgaagct tggctgtttt
     2641 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     2701 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     2761 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     2821 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2881 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     2941 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     3001 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt
     3061 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3121 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3181 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3241 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     3301 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3361 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     3421 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3481 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3541 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3601 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3661 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     3721 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3781 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3841 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3901 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3961 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4021 aagtttactc atatatactt tagattgatt tacgcgccct gtagcggcgc attaagcgcg
     4081 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4141 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4201 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4261 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4321 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4381 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4441 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4501 acaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4741 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
