LOCUS       sequence-162906-        3489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FAM173B, used as an over expression 
            control for gene expression analysis. Part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Berger AH, Brooks AN, Wu X, Shrestha Y, Chouinard C, Piccioni F, 
            Bagul M, Kamburov A, Imielinski M, Hogstrom L, Zhu C, Yang X, Pantel
            S, Sakai R, Watson J, Kaplan N, Campbell JD, Singh S, Root DE, 
            Narayan R, Natoli T, Lahr DL, Tirosh I, Tamayo P, Getz G, Wong B, 
            Doench J, Subramanian A, Golub TR, Meyerson M, Boehm JS
  TITLE     High-throughput Phenotyping of Lung Cancer Somatic Mutations.
  JOURNAL   Cancer Cell. 2016 Aug 8;30(2):214-28. doi: 
            10.1016/j.ccell.2016.06.022. Epub 2016 Jul 28.
  PUBMED    27478040
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.06.022"; journalName: "Cancer Cell"; date: 
            "2016-08-8- 8"; volume: "30"; issue: "2"; pages: "214-28"
COMMENT     Sequence Label: pDONR223_FAM173B_WT_V5
FEATURES             Location/Qualifiers
     source          1..3489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..59
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     293..310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(469..496)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(588..674)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    770..869
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1569..1668)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1685..1704)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1686..1704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1709..1725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1709..1725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2156..2947
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3040..3489,1..139)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
       61 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      301 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      361 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      421 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
      481 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
      541 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
      601 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
      661 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
      781 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
      841 aatgccaact ttgtacaaaa aagttggcat ggagggagga ggaggtatac ccctagaaac
      901 acttaaagaa gaaagtcagt caagacatgt tctacctgca agttttgaag tcaacagttt
      961 gcagaaaagc aactgggggt tcttacttac tgggcttgtg ggtggcaccc tggtggctgt
     1021 gtacgctgta gccacgccgt ttgtaacgcc agcccttcga aaagtctgtt tgccatttgt
     1081 acctgcaact atgaagcaga ttgaaaatgt tgtgaaaatg ttgcgatgcc gaagaggatc
     1141 ccttgtggac atcggtagtg gggacggacg cattgtcata gcggctgcga agaaagggtt
     1201 cacagcagtt ggttatgaat taaacccatg gctagtttgg tattccagat accgcgcttg
     1261 gcgagaaggt gtgcatggat ctgccaaatt ttatatttca gatttgtgga aggttacttt
     1321 ttcgcagtac tcgaacgttg ttattttcgg tgtgcctcag atgatgctgc agttggagaa
     1381 gaaacttgaa cgtgaacttg aggatgatgc acgagttatt gcttgccggt tccctttccc
     1441 acattggact ccagaccacg tcacggggga ggggatagac acagtgtggg catatgatgc
     1501 aagcactttt agaggccgtg aaaagaggcc ctgtacatcg atgcatttcc agctgcccat
     1561 tcaagcatac ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1621 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1681 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1741 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     1801 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     1861 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     1921 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1981 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     2041 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     2101 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     2161 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     2221 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2281 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2341 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2401 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     2461 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     2521 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     2581 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     2641 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     2701 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     2761 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     2821 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     2881 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     2941 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3001 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3061 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3121 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3181 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3241 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3301 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3361 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3421 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3481 ggtaagcgg
//
