LOCUS       sequence-162917-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Renilla SV40 promoter (no enhancer) reporter gene for normalization 
            of luciferase experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Chen X, Prywes R
  TITLE     Serum-induced expression of the cdc25A gene by relief of 
            E2F-mediated repression.
  JOURNAL   Mol Cell Biol. 1999 Jul . 19(7):4695-702.
  PUBMED    10373518
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Jul . 19(7):4695-702."
COMMENT     Sequence Label: pRL-SV40P
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(42..60)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(103..122)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          105..237
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(307..503)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      321..456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(663..682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgttctg gatcataaac tttcgaagtc atggtggcta gcctatagtg agtcgtatta
       61 agtactctag ccttaagagc tgtaattgaa ctgggagtgg acacctgtgg agagaaaggc
      121 aaagtggatg tcagtaagac caataggtgc ctatcagaaa cgcaagagtc ttctctgtct
      181 cgacaagccc agtttctatt ggtctcctta aacctgtctt gtaaccttga tacttacctg
      241 cccagtgcct cacgaccaac ttctgcagct taagttcgag actgttgtgt cagaagaatc
      301 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
      361 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc gatggggcgg
      421 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      481 ggttgctgac taattgagat gcagatcgca gatctgtcga gccatgtgag caaaaggcca
      541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      901 cccggtaaga cac
//
