LOCUS       sequence-163063-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to produce inducible SIV-derived vector. Cells tranduced
            by lentiviral particles will overexpress GFP upon activation of the 
            tetO promoter. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Mangeot PE, Dollet S, Girard M, Ciancia C, Joly S, Peschanski M, 
            Lotteau V
  TITLE     Protein transfer into human cells by VSV-G-induced nanovesicles.
  JOURNAL   Mol Ther. 2011 Sep;19(9):1656-66. doi: 10.1038/mt.2011.138. Epub 
            2011 Jul 12.
  PUBMED    21750535
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/mt.2011.138"; journalName: "Mol Ther"; date: "2011-09"; 
            volume: "19"; issue: "9"; pages: "1656-66"
COMMENT     Sequence Label: GAE teo-GFP
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(32..745,746..748,749..751))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(35..745,746..748,749..751))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(77..98)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     426..445
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     685..706
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(849..873)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(870..908)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    941..959
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 caaattttga atccagaggt tgattggatc ctcacttgta cagctcgtcc atgccgagag
       61 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
      121 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
      181 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
      241 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
      301 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
      361 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
      421 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
      481 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
      541 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
      601 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
      661 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
      721 cggtgaacag ctcctcgccc ttgctcacca tactggtggc gaccggtagc ttagccgggg
      781 ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
      841 ctccaggcga tctgacggtt cactaaacga gctctgctta tataggcctc ccaccgtaca
      901 cgcctacctc gacccgggta ccgagctcga ctttcacttt tctctatcac tgatagggag
      961 tggtaaactc gactttcact ttt
//
