LOCUS       sequence-163066-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Myc-tagged Mst2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Rawat SJ, Araiza-Olivera D, Arias-Romero LE, Villamar-Cruz O, 
            Prudnikova TY, Roder H, Chernoff J
  TITLE     H-ras Inhibits the Hippo Pathway by Promoting Mst1/Mst2 
            Heterodimerization.
  JOURNAL   Curr Biol. 2016 Jun 20;26(12):1556-63. doi: 
            10.1016/j.cub.2016.04.027. Epub 2016 May 26.
  PUBMED    27238285
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2016.04.027"; journalName: "Curr Biol"; date: 
            "2016-06-20- 20"; volume: "26"; issue: "12"; pages: "1556-63"
COMMENT     Sequence Label: pCMV6M-Mst2
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..110
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     RBS             779..787
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagaat taattcagat ctggtaccac gcgtatcgat
       61 aagcttcgat cgtcgacatg gaacagaaac tcatctctga agaggatctg tctagaggat
      121 ccgccatgga gcagccgccg gcgcctaaga gtaaactaaa aaagctgagt gaagacagtt
      181 tgactaagca gcctgaagaa gtttttgatg tattagagaa gcttggagaa gggtcttatg
      241 gaagtgtatt taaagcaata cacaaggaat ccggtcaagt tgtcgcaatt aaacaagtac
      301 ctgttgaatc agatcttcag gaaataatca aagaaatttc cataatgcag caatgtgaca
      361 gcccatatgt tgtaaagtac tatggcagtt attttaagaa tacagacctc tggattgtta
      421 tggagtactg tggcgctggc tctgtctcag acataattag attacgaaac aagacattaa
      481 tagaagatga aattgcaacc attcttaaat ctacattgaa aggactagaa tatttgcact
      541 ttatgagaaa aatacacaga gatataaaag ctggaaatat tctcctcaat acagaaggac
      601 atgcaaaatt ggcagatttt ggagtggctg gtcagttaac agatacaatg gcaaaacgca
      661 atactgtaat aggaactcca ttttggatgg ctcctgaggt gattcaagaa ataggctata
      721 actgtgtggc cgacatctgg tcccttggca ttacttctat agaaatggct gaaggaaaac
      781 ctccttatgc tgatatacat ccaatgaggg ctattt
//
