LOCUS       sequence-163084-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting murine CD45.2 region 3. Co-expresses 
            SpCas9-2A-EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Kornete M, Marone R, Jeker LT
  TITLE     Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary
            T Cells.
  JOURNAL   J Immunol. 2018 Feb 14. pii: jimmunol.1701121. doi: 
            10.4049/jimmunol.1701121.
  PUBMED    29445007
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol. 
            2018 Feb 14. pii: jimmunol.1701121. doi: 10.4049/jimmunol.1701121."
COMMENT     Sequence Label: p206_LTJ_sgRNACD45.2_R3
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        48..123
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        220..505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tatatcttgt ggaaaggacg aaacaccgta ggtccggaca aggtcaagtt ttagagctag
       61 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      121 tgcttttttg ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg
      181 cgtgcgccaa ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat
      301 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      361 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      421 cgcctggcat tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      481 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      541 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      601 agcgatgg
//
