LOCUS       sequence-163108-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same 
            white sgRNA-1 from a U6:1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ge DT, Tipping C, Brodsky MH, Zamore PD
  TITLE     Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome
            Using white Co-conversion.
  JOURNAL   G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557.
  PUBMED    27543296
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557."
COMMENT     Sequence Label: pCFD4d-U6-1:white1-U6-3:white1
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..832
                     /label=dU6-1 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-1 
                     snRNA (Port et al., 2014)"
     misc_RNA        853..928
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tatagggcga attgggtaca agctgatctg aattcatttt caacgtcctc gatagtatag
       61 tggttagtat ccccgcctgt cacgcgggag accggggttc aattccccgt cggggagaat
      121 ctgtgattct tttttttttt cttttacttt gttatataaa caatttttgt tttaattgaa
      181 tctaatttgc cattgctttt aggaatctca ggcatccagc aagcgtttgt ccgccgaatc
      241 gcccatcagt gaagaagatc ctgtggcggc tacgaaaatc tccccggcca tgtcggcctc
      301 cacctccagc gaaaaaccca tcagcgagct ggccacctct gtgctgaccc accgctttcc
      361 agactccacc tcctcacccg gcgaacatgg ccttggacga atgcagttgt cgatccgcta
      421 cagcgcccag cgtcaaaaac tagacgtgac catacacaaa atccagaaga taccacttcg
      481 cgatcccagc aatatccccg atccgtatgt taagctgtat ctgttgcctg gacgcaccaa
      541 ggagtcgaaa cgcaagacga gcgtgatcaa ggacaactgc aaccccgtct acgatgcatc
      601 ctttgagtac ctgatttcca ttgccgaact caggcagacg gaactggagg tgacggtgtg
      661 cacccaaaag ggattcctat ccggcggtag tcccatcatt ggcatggtag gtacccgaaa
      721 gcaacccctt agttacagac acagcgcgta cgtccttcgc atccttatga ttcccaagta
      781 catattctgc aagagtacag tatatatagg aaagatatcc gggtgaactt cgataccatt
      841 cctgctcttt gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      901 acttgaaaaa gtggcaccga gtcggtgctt ttttgctcac ctgtgattgc tcctactcaa
      961 atacaaaaac atcaaatttt ctgtcaataa agcatattta tttatatt
//
