LOCUS       sequence-163119-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor HR template carrying a 2,080 bp fragment of the Drosophila 
            melanogaster white[coffee] allele and silent mutations conferring 
            resistance to white sgRNAs-1, -2, -3, and -4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Ge DT, Tipping C, Brodsky MH, Zamore PD
  TITLE     Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome
            Using white Co-conversion.
  JOURNAL   G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557.
  PUBMED    27543296
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557."
COMMENT     Sequence Label: pUC57-white[coffee]
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cactccgaac aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat
       61 atagctgtga gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat
      121 ttattactaa tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat
      181 cgcgcggaac taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga
      241 gagcgggtgg gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat
      301 gaagagggcg cgcgatttga aaagtttgta tataaaaaat atatcccggt gttttatgta
      361 gcgataaacg agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca
      421 aatccaaagt ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata
      481 tcttgttttt attggcacaa atataattac attagctttt tgagggggca ataaacagta
      541 aacacgatgg taataatggt aaaaaaaaaa caagcagtta tttcggatat atgtcggcta
      601 ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat
      661 gagaatggcc agacccacgt agtccagcgg cagatcggcg gccgagaagt taagcgtctc
      721 caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc
      781 cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga
      841 gaggtacgac aaccatttga ggtaaactgg caccgagccc gagttcaaga agaat
//
