LOCUS       sequence-163280-        4995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Gli3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4995)
  TITLE     Fernandez-Zapico lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: hGli3 6x his
FEATURES             Location/Qualifiers
     source          1..4995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(4931..4948)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggtaccag gatccagtgt ggtggaattc
      121 tgcagatatc cagcacagtg gcggccgctc gagtctagaa tggaggccca gtcccacagc
      181 tccacgacca ctgaaaagaa aaaagttgag aattccatag tgaagtgctc cactcgaaca
      241 gatgtgagcg agaaagccgt tgcctccagc accacttcta atgaggatga aagtcctgga
      301 cagacttatc acagagagag aagaaacgca atcactatgc agccacagaa tgtccagggg
      361 ctcagcaaag tcagtgagga accttcaaca tcgagtgacg agagggcctc attgatcaag
      421 aaagagatcc atgggtccct gccacacgtg gcggagccct ctgtgccgta ccgcgggacg
      481 gtgtttgcca tggaccccag gaatggttac atggagcccc actaccaccc tcctcatctt
      541 ttccctgcct tccatcctcc tgtaccaatt gatgccagac atcatgaggg ccgttaccat
      601 tacgatccat ctccgattcc tccattgcat atgacttccg ccttatctag tagccctacg
      661 tatccggacc tgcccttcat taggatctcc ccacaccgga accccactgc tgcttccgag
      721 tctcccttca gccctccaca tccctacatt aatccctaca tggactatat ccgctccttg
      781 cacagcagcc catcgctctc catgatctca gcaacccgtg ggctgagccc tacagatgcg
      841 ccccatgcag gagtcagccc agcagaatac tatcatcaga tggccctgct aactggccag
      901 cgcagcccct atgcagacat tattccctca gctgccaccg ccggcacggg ggccatccac
      961 atggaatatc ttcatgctat ggatagcacc agattctcca gccccaggct gtcagccagg
     1021 ccgagccgaa aacgtacact gtccatatca ccactctccg atcatagctt tgaccttcag
     1081 accatgataa ggacgtctcc caactccttg gtcacgattc tcaataattc ccgtagcagc
     1141 tcttcagcaa gtggctccta tggtcactta tctgcaagtg caatcagccc tgccttgagc
     1201 ttcacctact cttccgcgcc cgtctctctc cacatgcatc agcagatcct aagccgacaa
     1261 cagagcttag gttcagcctt tggacacagc cctccactca tccaccctgc cccaactttt
     1321 ccaacacaga ggcctattcc agggatccct acggttctga accccgtcca ggtcagctcc
     1381 ggcccttctg agtcctcaca gaacaagccc acgagtgagt ctgcagtgag cagcactggt
     1441 gacccgatgc acaacaagag gtccaagatc aaacccgatg aagacctccc cagcccaggg
     1501 gctcgggggc agcaggaaca gcccgaagga acaacccttg tcaaggagga aggggacaaa
     1561 gatgaaagca aacaggagcc tgaagtcatc tatgagacaa actgccactg ggaaggctgc
     1621 gcgagggagt tcgacaccca agagcagctt gtgcaccata taaataacga ccatattcat
     1681 ggagagaaga aggagttcgt gtgcaggtgg ctggactgct caagagagca gaaacccttc
     1741 aaagcccagt atatgttggt agtgcatatg agaagacaca cgggcgagaa gcctcacaaa
     1801 tgcacttttg aaggttgcac aaaggcctac tcgagactag aaaacttgaa aacacacttg
     1861 agatctcaca ctggagagaa accatacgtc tgtgagcacg aaggttgcaa caaggctttc
     1921 tcaaatgcct ctgatcgcgc caaacaccaa aacagaacgc attccaatga gaaaccatat
     1981 gtgtgcaaaa tcccaggctg cactaagcgt tacacagacc caagctccct ccggaaacat
     2041 gtgaagacag tgcatggccc agaggctcat gtcaccaaga agcagcgagg ggacatccat
     2101 cctcggccgc cacccccgag agattccggc agccattcac agtccaggtc gcctggccga
     2161 ccgactcagg gagcccttgg tgagcagcag gacctcagca acactacctc aaagcgggaa
     2221 gaatgcctcc aggtgaaaac cgtcaaggca gagaagccaa tgacatctca gccaagccct
     2281 ggtggtcagt cttcatgcag cagccaacag tcccccatca gcaactattc caacagtggg
     2341 ctcgagcttc ctctgaccga tggaggtagt ataggagacc tcagtgccat cgatgaaacc
     2401 ccaatcatgg actcaaccat ttccactgca accacagccc ttgctttgca agccaggaga
     2461 aacccggcag ggaccaaatg gatggagcac gtaaaactag aaaggctaaa acaagtgaat
     2521 ggaatgtttc cgcgactgaa ccccattcta ccccctaaag cccctgcggt ctctcctctc
     2581 ataggaaatg gcacacagtc caacaacacc tgcagcttgg gtgggcccat gacgcttctc
     2641 ccgggcagaa gcgacctctc tggggtggac gtcactatgc tgaacatgct caacagaagg
     2701 gacagcagcg ccagcaccat cagctcggcc tacctgagca gccgccgctc ctcagggatc
     2761 tcgccctgct tctccagccg ccgctccagc gaggcgtcac aggccgaggg ccggccgcag
     2821 aacgtgagcg tggccgactc ctacgacccc atctccaccg acgcctcgcg ccgctccagc
     2881 gaagccagcc agagcgacgg cctgcccagc ctgctcagcc tcacgcccgc ccagcagtac
     2941 cgcctcaagg ccaagtacgc ggctgccaca ggagggccgc cgccgacgcc cctgcccaac
     3001 atggagagga tgagcctgaa gacgcgcctg gcgctgctcg gggatgccct cgagcctggc
     3061 gtggccctgc ctccagttca tgccccgagg aggtgcagcg acgggggagc ccacggctac
     3121 gggcggcgcc acctgcagcc gcacgatgcg ctgggccacg gcgtgaggag ggccagcgac
     3181 ccggtgcgga caggctccga gggcctggcc ctgcctcgtg tgccgcgctt cagcagcctc
     3241 agcagctgca accccccggc gatggccacg tccgcggaga agcgcagtct cgtgcttcag
     3301 aattacacgc ggcccgaggg cggccagtcc cgaaacttcc actcgtcccc ctgtcctccc
     3361 agcatcaccg agaacgtcac cctggagtcc ctgaccatgg acgctgatgc caacctgaac
     3421 gatgaggatt tcctgccgga cgacgtggtg cagtatttaa attcccagaa ccaagcaggg
     3481 tacgagcagc acttccccag cgccctcccg gacgacagca aagtgcccca cgggcccggt
     3541 gactttgacg cgcccgggct gccagacagc cacgctggcc agcagttcca tgccctcgag
     3601 cagccctgcc ccgagggcag caaaaccgac ctgcccattc agtggaacga agtcagctcc
     3661 ggaagcgccg acctgtcctc ctccaagctc aagtgtgggc cgcggcccgc tgtgccgcag
     3721 actcgcgcct ttgggttctg caacggcatg gtcgtccacc cgcagaaccc cttgaggagc
     3781 gggcctgctg ggggctatca gaccctcggg gagaacagca acccctacgg tggcccagag
     3841 cacttgatgc tccacaacag ccccggaagt ggcaccagtg gaaacgcctt ccatgaacag
     3901 ccctgtaagg ccccgcagta tgggaactgt ctcaacaggc agccagtggc ccctggtgca
     3961 ctcgacggtg cctgtggtgc cgggattcaa gcctcaaagc tgaagagcac ccccatgcaa
     4021 gggagcgggg gccagctgaa tttcggcctg ccggtagcgc caaatgagtc agctggcagc
     4081 atggtgaatg gcatgcagaa ccaggaccca gtgggacagg ggtacctggc tcaccagctc
     4141 ctcggcgaca gcatgcagca cccgggggca ggccgccccg gtcagcagat gcttgggcag
     4201 attagtgcta cctcacacat caacatctac caagggccag agagctgcct gccaggggct
     4261 cacggcatgg gcagccagcc gtcaagcttg gcagttgtca ggggctacca gccatgtgcc
     4321 agctttgggg gcagcaggcg ccaggctatg ccgagggaca gccttgctct gcagtcagga
     4381 cagctcagtg acacaagtca gacctgcagg gtgaatggta tcaagatgga gatgaaaggg
     4441 cagccccatc cgctgtgctc taatctgcag aattactctg gtcagttcta tgaccaaacc
     4501 gtgggcttca gtcagcaaga cacgaaagct ggttcattct ctatttcaga cgccagctgc
     4561 ctgctacagg ggaccagcgc caaaaactct gagttacttt ccccaggtgc taatcaggtg
     4621 acaagcacag tggacagcct cgacagccat gacctggaag gggtacagat tgacttcgat
     4681 gccatcatag acgatgggga ccactccagc ctgatgtcgg gggccctgag cccaagtatc
     4741 attcagaacc tttcccatag ctcctcccgc ctcaccacgc ctcgggcgtc cctcccattc
     4801 ccagcgctgt ccatgagcac caccaacatg gctatcgggg acatgagttc tttgctgacc
     4861 tccctagcgg aagaaagcaa attccttgca gttatgcaat ctagagggcc cgtttaaacc
     4921 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     4981 gtgccttcct tgacc
//
