LOCUS       sequence-163485-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the nDHFR-link1-His6-Ub, UBC1 Sc and UBA1 Ta in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pND-Ub01-UBC1 Sc
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaaaacactg ccaaaaagac cacgaacttc agacttgatg aacgcaatgg gtggctgttg
       61 gctgtggcag taatcatcaa attcaattgc tttatctaag ctgatatcag tgaagacaac
      121 ggcctggaat ttagaaaggt gctccttggt caaatcaccg gttaaggcag agacgaggac
      181 agcattgttc agctcttgta gcttctgtac acaagcttga gcacggtttt gcccaacatc
      241 attctccgag aggaagaagt tgcttgataa gtcccacagc tccacgttac catcatcatg
      301 caaggttaca gacttgacac ccgcaaggac aaggttcttt gcgatctcag caccgagacc
      361 ctggagtcca gagacgagga cgttggagcc gaagaggcgc ttcatggtct cgcgcccata
      421 gacggcgagc tggcggctgt gcaggtcctc gtcgatctcg ttccccctcc ctgccatggc
      481 tgcgtctcct tcctccctag tgacctcccc ctccccggcg cgggtcttct tctgcaagtc
      541 ctcgacctcg ccggcgacga tctcccgctt ccggggaagc atagctgttt cctgtcggaa
      601 ttgtggcgcg cctcacttca acaattcctc gatgatacgg ttggctgtgt tgttgtcatt
      661 ggggtctaag gacttgacgc ctaatcgtct caacacttcc acaatcttgt ccttttcaaa
      721 accttgagat tcaaactcgt caatcagatc gtggtcaatc ccatataaat cagactcctc
      781 tacattgcct ttttgaccat tggaagtttc actggcgtat aacctcgtcc atagtgcagc
      841 agtctta
//
