LOCUS       sequence-163514-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR1-HA Cterm
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        192..210
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     192..209
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     223..242
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg
       61 tgcattggaa cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat
      121 aggcccaccc ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc
      181 atacacatac gatttaggtg acactataga ataacatcca ctttgccttt ctctccacag
      241 gtgtccactc ccaggtccaa ctgcacctcg gttctatcga ttgaattcgt cgacgccacc
      301 atggagctgc acatcctaga acaccgggtg cgggtgctga gcgtcgcccg tcccggtctc
      361 tggctctaca cccacccgct catcaagctg ctcttcctgc cccgccgcag ccggtgcaag
      421 ttcttcagcc tgacggagac ccctgaggat tacacgctta tggtggacga ggagggcttt
      481 aaagagctgc ccccatctga gttcctgcaa gtagctgagg ccacatggct ggtgctgaac
      541 gtgtcgtctc acagcggtgc ggcagtgcag gctgctgggg tcaccaagat cgcccgttcg
      601 gtcatcgcgc cactggccga gcaccacgtg tctgtgctga tgctgtccac ttaccagacg
      661 gacttcatcc tggtgcggga gcaggacctg tccgtggtga tccacacgct ggcccaggag
      721 ttcgacattt accgcgaggt gggcggagag cctgtgcctg tgacgaggga tgattccagc
      781 aatggctttc cccgcactca gcatgggccc agccccacgg tgcatcccat ccagagccca
      841 cagaaccgct tctgtgtcct cacactggac cctgagacgc ttccagccat cgccaccacc
      901 ctcatagatg tcctcttcta ctcgcacagc acccccaagg aggcagcctc tagcagtcct
      961 gaacccagct ccatcacgtt ctttgccttc t
//
