LOCUS       sequence-163515-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of CASTOR1 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Chantranupong L, Scaria SM, Saxton RA, Gygi MP, Shen K, Wyant GA, 
            Wang T, Harper JW, Gygi SP, Sabatini DM
  TITLE     The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway.
  JOURNAL   Cell. 2016 Mar 24;165(1):153-64. doi: 10.1016/j.cell.2016.02.035. 
            Epub 2016 Mar 10.
  PUBMED    26972053
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.02.035"; journalName: "Cell"; date: 
            "2016-03-24- 24"; volume: "165"; issue: "1"; pages: "153-64"
COMMENT     Sequence Label: pRK5 CASTOR1-HA Cterm
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(37..63)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gttgggccat ggcggccaag ctggggatcc ttatcatgcg tagtcaggca catcataggg
       61 gtaacctcca cctcctgcgg ccgcggaagc caggccttcc tgccgccgct ggaggacctc
      121 gatgacgctg ccgataccgt cctcgggcac cagggcgtgg tcgaagttga aggtgctgat
      181 gtagtaggca gagatgtcag cggcagccag gggacctgca atctgtgcca cgatgccaca
      241 ttcatcaaag cccaggggct gtccaccgat gcgcaccatc ctccacagct cccccgagga
      301 gctggtcagc aggaggtcac tggggaactt tttctgtgtt tcagcatcca tgacaatgga
      361 gatataaccc tcgatgagag agaaggcaaa gaacgtgatg gagctgggtt caggactgct
      421 agaggctgcc tccttggggg tgctgtgcga gtagaagagg acatctatga gggtggtggc
      481 gatggctgga agcgtctcag ggtccagtgt gaggacacag aagcggttct gtgggctctg
      541 gatgggatgc accgtggggc tgggcccatg ctgagtgcgg ggaaagccat tgctggaatc
      601 atccctcgtc acaggcacag gctctccgcc cacctcgcgg taaatgtcga actcctgggc
      661 cagcgtgtgg atcaccacgg acaggtcctg ctcccgcacc aggatgaagt ccgtctggta
      721 agtggacagc atcagcacag acacgtggtg ctcggccagt ggcgcgatga ccgaacgggc
      781 gatcttggtg accccagcag cctgcactgc cgcaccgctg tgagacgaca cgttcagcac
      841 cagccatgtg gcctcagcta cttgcaggaa ctcagatggg ggcagctctt taaagccctc
      901 ctcgtccacc ataagcgtgt aatcctcagg ggtctccgtc aggctgaaga acttgcaccg
      961 gctgcggcgg ggcaggaaga gcagc
//
