LOCUS       sequence-163573-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78C protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78C
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             373..395
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    410..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(434..453)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(435..453)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(537..556)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        762..839
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(859..878)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcgt ttagttaatc agtgatgatg gtgatggtgg
       61 gatccctgag acggtttgtc gatttcggtg cgataattga tgctgattgg gtttgaatcg
      121 tcgttacaac cggttacagc gtacacggta atggtataat cctggcccgg tttcagaccg
      181 ctgatggtcg cattagaata agatctcgga acagtgaatt cctggctcgg ggagttacca
      241 ccagtttcgc cgtaggtgat acggtaatag atagcgtgat aaggatcgtc ccaagaaata
      301 gtcagagagt tcggagttgc gttggtaacc tccaggttac gcggagagtc ggaggagcta
      361 gccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      421 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      481 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      541 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
      601 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
      661 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
      721 cctaatgcag gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca
      781 aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg
      841 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
      901 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgg
//
