LOCUS       sequence-163615-        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78G protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78G
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             344..361
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(426..444)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      440..487
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      524..979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(611..630)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     821..842
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg gctagctcct ccgactctcc
       61 gcgtaacctg gaggttacca acgcaactcc gaactctctg actatttctt gggacgatcc
      121 ttatcacgct atctattacc gtatcaccta cggcgaaact ggtggtaact ccccgagcca
      181 ggaattcact gttccgagat cttattctaa tgcgaccatc agcggtctga aaccgggcca
      241 ggattatacc attaccgtgt acgctgtaac cggtgggaac gacgattcaa acccaatcag
      301 catcaattat cgcaccgaaa tcgacaaacc gtctcaggga tcccaccatc accatcatca
      361 ctgattaact aaacgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc
      421 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
      481 ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc
      541 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      601 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      661 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      721 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      781 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      841 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      901 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      961 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1021 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaa
//
