LOCUS       sequence-163620-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of FnR.0.6.2-Y78H protein .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Johnson SM, Javner C, Hackel BJ
  TITLE     Development and Implementation of a Protein–Protein Binding 
            Experiment To Teach Intermolecular Interactions in High School or 
            Undergraduate Classrooms
  JOURNAL   J. Chem. Educ. 2017, 94, 3, 367–374
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Chem. 
            Educ. 2017, 94, 3, 367–374"
COMMENT     Sequence Label: pET - FnR062.Y78H
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             357..379
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    394..418
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(418..437)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(419..437)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(521..540)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        746..823
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(843..862)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cagccggatc tcgtttagtt aatcagtgat gatggtgatg gtgggatccc tgagacggtt
       61 tgtcgatttc ggtgcgataa ttgatgctga ttgggtttga atcgtcgtta tgaccggtta
      121 cagcgtacac ggtaatggta taatcctggc ccggtttcag accgctgatg gtcgcattag
      181 aataagatct cggaacagtg aattcctggc tcggggagtt accaccagtt tcgccgtagg
      241 tgatacggta atagatagcg tgataaggat cgtcccaaga aatagtcaga gagttcggag
      301 ttgcgttggt aacctccagg ttacgcggag agtcggagga gctagccata tgtatatctc
      361 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
      421 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
      481 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
      541 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
      601 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
      661 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
      721 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
      781 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
      841 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
      901 ggccagccac gtttctgcga aaacgcggg
//
