LOCUS       sequence-163804-        4398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SRC , part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SRC_WT
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1798..1897)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1914..1933)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1915..1933)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1938..1954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1938..1954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2385..3176
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3269..3857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3758..3777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4011..4028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4187..4214)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4306..4392)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gggtagcaac aagagcaagc ccaaggatgc cagccagcgg cgccgcagcc
      241 tggagcccgc cgagaacgtg cacggcgctg gcgggggcgc tttccccgcc tcgcagaccc
      301 ccagcaagcc agcctcggcc gacggccacc gcggccccag cgcggccttc gcccccgcgg
      361 ccgccgagcc caagctgttc ggaggcttca actcctcgga caccgtcacc tccccgcaga
      421 gggcgggccc gctggccggt ggagtgacca cctttgtggc cctctatgac tatgagtcta
      481 ggacggagac agacctgtcc ttcaagaaag gcgagcggct ccagattgtc aacaacacag
      541 agggagactg gtggctggcc cactcgctca gcacaggaca gacaggctac atccccagca
      601 actacgtggc gccctccgac tccatccagg ctgaggagtg gtattttggc aagatcacca
      661 gacgggagtc agagcggtta ctgctcaatg cagagaaccc gagagggacc ttcctcgtgc
      721 gagaaagtga gaccacgaaa ggtgcctact gcctctcagt gtctgacttc gacaacgcca
      781 agggcctcaa cgtgaagcac tacaagatcc gcaagctgga cagcggcggc ttctacatca
      841 cctcccgcac ccagttcaac agcctgcagc agctggtggc ctactactcc aaacacgccg
      901 atggcctgtg ccaccgcctc accaccgtgt gccccacgtc caagccgcag actcagggcc
      961 tggccaagga tgcctgggag atccctcggg agtcgctgcg gctggaggtc aagctgggcc
     1021 agggctgctt tggcgaggtg tggatgggga cctggaacgg taccaccagg gtggccatca
     1081 aaaccctgaa gcctggcacg atgtctccag aggccttcct gcaggaggcc caggtcatga
     1141 agaagctgag gcatgagaag ctggtgcagt tgtatgctgt ggtttcagag gagcccattt
     1201 acatcgtcac ggagtacatg agcaagggga gtttgctgga ctttctcaag ggggagacag
     1261 gcaagtacct gcggctgcct cagctggtgg acatggctgc tcagatcgcc tcaggcatgg
     1321 cgtacgtgga gcggatgaac tacgtccacc gggaccttcg tgcagccaac atcctggtgg
     1381 gagagaacct ggtgtgcaaa gtggccgact ttgggctggc tcggctcatt gaagacaatg
     1441 agtacacggc gcggcaaggt gccaaattcc ccatcaagtg gacggctcca gaagctgccc
     1501 tctatggccg cttcaccatc aagtcggacg tgtggtcctt cgggatcctg ctgactgagc
     1561 tcaccacaaa gggacgggtg ccctaccctg ggatggtgaa ccgcgaggtg ctggaccagg
     1621 tggagcgggg ctaccggatg ccctgcccgc cggagtgtcc cgagtccctg cacgacctca
     1681 tgtgccagtg ctggcggaag gagcctgagg agcggcccac cttcgagtac ctgcaggcct
     1741 tcctggagga ctacttcacg tccaccgagc cccagtacca gcccggggag aacctctacc
     1801 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1861 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1921 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1981 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2041 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2101 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2161 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2221 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2281 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2341 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2401 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2461 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2521 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2581 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2641 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2701 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2761 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2821 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2881 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2941 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3001 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3061 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3121 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3181 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3241 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3301 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3361 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3421 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3481 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3541 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3601 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3661 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3721 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3781 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3841 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3901 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3961 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4021 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4081 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4141 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     4201 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4261 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4321 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4381 tcgttttatt tgatgcct
//
