LOCUS       sequence-163974-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..25)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atcacacctt cctcttcttc ttggggtcag ccctgctgtc tccaccgagc tgagagaggt
       61 cgattcttgt ttcatagagc cccgtaattg actgatgaat cagtgtggcg tccaggacct
      121 cctttgtaga ggtgtaccgc tttctgtcta tggtggtgtc gaagtacttg aaggctgcag
      181 gcgcgcccaa gttggtcaga gtaaacaagt ggataatgtt ttctgcctgc tccctgatgg
      241 gcttatccct gtgcttattg taagcagaaa gcaccttatc gaggttagcg tcggcgagga
      301 tcactctttt ggagaattcg cttatttgct cgatgatctc atcaaggtag tgtttgtgtt
      361 gttccacgaa cagctgcttc tgctcattat cttcgggaga ccctttgagc ttttcatagt
      421 ggctggccag atacaagaaa ttaacgtatt tagagggcag tgccagctcg ttacctttct
      481 gcagctcgcc cgcactagcg agcattcgtt tccggccgtt ttcaagctca aagagagagt
      541 acttgggaag cttaatgatg aggtcttttt tgacctcttt atatcctttc gcctcgagaa
      601 agtcgatggg gtttttttcg aagcttgatc gctccatgat tgtgatgccc agcagttcct
      661 tgacgctttt gagtttttta gacttccctt tctccacttt ggccacaacc agtacactgt
      721 aagcgactgt aggagaatcg aatccgccgt atttcttggg gtcccaatct tttttgcgtg
      781 cgatcagctt gtcgctgttc cttt
//
