LOCUS       sequence-163996-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Jackman M, Kubota Y, den Elzen N, Hagting A, Pines J
  TITLE     Cyclin A- and cyclin E-Cdk complexes shuttle between the nucleus and
            the cytoplasm.
  JOURNAL   Mol Biol Cell. 2002 Mar;13(3):1030-45.
  PUBMED    11907280
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2002-03"; volume: "13(3)"; pages: "1030-45"
COMMENT     Sequence Label: pcDNA3/CyclinB1-GFP (28)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..811)
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(141..161)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     756..784
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatatctgc
       61 agaattccag cacactggcg gccgttacta gtggatccta tttgtatagt tcatccatgc
      121 catgtgtaat cccagcagcc gttacaaact caagaaggac catgtggtct ctcttttcgt
      181 tgggatcttt cgaaagggca gattgtgtgg acaggtaatg gttgtctggt aaaaggacag
      241 ggtcatcgcc aattggagta ttttgttgat aatgatcagc gagttgcacg ccgccgtctt
      301 cgatgttgtg gcgggtcttg aagttggctt tgatgccgtt cttttgcttg tcggccatga
      361 tgtatacgtt gtgggagttg tagttgtatt ccaacttgtg gccgaggatg tttccgtcct
      421 ccttgaaatc gattccctta agctcgatcc tgttgacgag ggtgtctccc tcaaacttga
      481 cttcagcacg tgtcttgtag ttcccgtcgt ccttgaagaa gatggtcctc tcctgcacgt
      541 atccctcagg catggcgctc ttgaagaagt cgtgccgctt catatgatct ggatatcttg
      601 aaaagcattg aacaccataa gtgagagtag tgacaagtgt tggccatgga acaggtagtt
      661 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct
      721 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga
      781 caactccagt gaaaagttct tctcctttac tgaattgcgc tcctgccacc tttgccacag
      841 ccttggctaa
//
