LOCUS       sequence-164028-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger 
            K
  TITLE     A high-efficiency system for the generation and study of human 
            induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
  PUBMED    18786420
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2008 Sep 11. 3(3):340-5."
COMMENT     Sequence Label: FUdeltaGW-rtTA
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(173..790,791..796,797..832,833..835,836..871,872..874,
                     875..910,911..919)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(1001..1021)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
       61 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
      121 tttgttagac gaagcttggg ctgcaggtcg actctagaat taattcgcca ccatgtctag
      181 actggacaag agcaaagtca taaacggcgc tctggaatta ctcaatggag tcggtatcga
      241 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
      301 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
      361 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
      421 caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
      481 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
      541 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
      601 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
      661 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
      721 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
      781 cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga
      841 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
      901 tgacatgctc cccgggtaaa ctagaattcg atatcaagct tatcgataat caacctctgg
      961 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1021 gtggatacgc tgctttaat
//
