LOCUS       sequence-164093-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YFP-CFP positive FRET control; his selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Hailey DW, Davis TN, Muller EG
  TITLE     Fluorescence resonance energy transfer using color variants of green
            fluorescent protein.
  JOURNAL   Methods Enzymol. 2002;351:34-49.
  PUBMED    12073355
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2002"; volume: "351"; pages: "34-49"
COMMENT     Sequence Label: pDH18
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..75
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     57..74
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             136..852
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             136..852
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     792..812
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
       61 aggtgacact atagaacgcg gccgccagct gaagcttcgt acgctgcagg tcgacggatc
      121 cccgggttaa ttaacatggt gagtaaagga gaagaacttt tcactggagt tgtcccaatt
      181 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      241 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      301 gttccatggc caacacttgt cactactttc ggttatggtc taaaatgctt tgctagatac
      361 ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      421 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      481 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      541 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      601 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      661 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      721 ttaccagaca accattacct gtcctatcaa tctgcccttt cgaaagatcc caacgaaaag
      781 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      841 gaactataca aaggcgccag taaaggagaa gaacttttca ctggagttgt cccaattctt
      901 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtgga
//
