LOCUS       sequence-164123-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRICK translation biosensor with 12x copies of modified PP7 stem 
            loops and 24x MS2 stem loops..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: pmax pona 12xTRICK 24xMS2SL
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             139..165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             172..198
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtaccgaagc cgctagcgct accggtcgcc accatgtcgc ggaggcggca cagctacgag
       61 aacgatggtg gacaacctca caaaaggagg aagacgtctg ttgagtaccc ttatgatgtc
      121 cctgactacg ctgccgagta tccctacgac gtccccgatt atgccgcaga atacccatac
      181 gatgtgccag actacgcagc tgaggggggg caagtccagt tgcaagaatc tggacctggt
      241 ttggttaaac catctgagac actttcccta acctgcacag tcagtggcgc atcaatttcc
      301 tcctcgcact attattgggg ttggattaga caaccacctg gaaagggccc agagtggata
      361 gggtctatgt actactcggg tagaacttac tataatcctg ccttgaagtc acgtgttacc
      421 atatcctctg ataagtcaaa aaaccaattc tttttaaaac tcactagcgt taccgctgct
      481 gatacagctg tctattatgc tgcccgtgag ggcccaacgc attattatga taattcaggc
      541 ccaataccta gcgatgagta ttttcaacat tggggccagg gcactttagt tacagtttcc
      601 ggtggtggcg gctctggtgg cggtggcagc ggcggtggtg gttccggagg cggcggttct
      661 caggtgcagc tgcaggagtc gggcccagga ctggtgaagc cttcggagac cctgtccctc
      721 acctgcactg tctctggtgc ctccatcagc agtagtcatt actactgggg ctggattcgc
      781 cagcccccag ggaaggggcc tgagtggatt gggagtatgt attatagtgg gagaacgtac
      841 tacaacccgg ccctcaagag tcgagtcacc atatcatcag acaagtcgaa gaaccagttc
      901 ttcttgaagt tgacctctgt aaccgccgcg gacacggccg tgtattacgc tgcgagggag
      961 ggacccacac attactatga taatagtggt ccaatacctt cggatgagta tttccag
//
