LOCUS       sequence-164149-         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-2
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(44..292)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(102..121)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(272..292)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    343..460
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(758..802)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgctatttc tagctctaaa acagcggagc cgcgagaagt gccggtgttt cgtcctttcc
       61 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      121 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      181 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
      241 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcggtacctt
      301 aattaaccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
      361 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
      421 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
      481 ttgtctgcag aattggcgca cgcagtgccg atccgttcac taatcgaatg gatctgtctc
      541 tgtctctctc tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg
      601 ggaggtgggt ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa
      661 gtacagcaaa aactattctt aaacctacca agcctcctac tatcattatg aataatttta
      721 tataccacag ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt
      781 ccaataattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta
      841 ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa
      901 tctgttcca
//
