LOCUS       sequence-164203-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCOG40-HRS (257-276) Hs
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(182..202)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(215..868)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(235..257)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(880..902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    891..907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(915..921,922..937,938..943))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 cgcgcgaggc agatcgtcag tcagtcacga tgcggccgct cgagactgca ggctctagat
       61 tcgaaagcgg ccgcgactag tgagctcgtc gacgtaggcc tttgaattct tattccgctt
      121 cagactgaga gagcgccaga gccagctgca gttcttcctc ttcctgcagg gatcccatgg
      181 cgccctgaaa atacaggttt tcggtcgtat cgatttttgg aggatggtcg ccaccaccaa
      241 acgtggcttg ccagccctgc aaaggccatg ctatatactt gctggatttc aagtacttat
      301 caatttgtgg gatagcttca atacgttttt taaaacaaac taattttggg aacgcatcca
      361 ggcacattgg gtccatgtat aaaacaacat caagagcgtc atacaacatg aagtcaggat
      421 gggttacatg atcaccattt aaatatgttt tatgacataa acgatcttcg aacattttca
      481 gcatttcagg tagcttgcta agaaaatcaa ctttgagagt ttcaaagtct ttactatatg
      541 caattctcga aacaccgtat ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg
      601 cacgctcttt tggacaacca cccaacatgt tgtgcttgtc agctatataa cgtatgatgg
      661 ccatagactg tgttaattta acatcaccat caatataata aggaagattg ggaaactcca
      721 aacccaattc aaactttttg tttcgccatt tatcaccttc atcgcgctca tacaaatgct
      781 cttcatattt ttcttcaaga tattccaaaa gaagtcgagt gggttgcaca aggcccttaa
      841 ttttccaata acctagtata ggggacatga atactgtttc ctgtgtgaaa ttgttatccg
      901 ctcacaattc cacacattat acgagccgat gattaattgt caacagctca tttcagaata
      961 tttgccagaa ccgttatgat gtcggcgcaa aaaacattat ccagaacgg
//
