LOCUS       sequence-164214-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHis-par2-UbHs/Ubc5Sc/ Uba1Ta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pGEN6-Ubc5 Sc
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(918..929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagactg caggctctag
       61 attcgaatca gcggaagtag accgacacaa gtgggatgtc gacatcattg tcgtcgtcat
      121 cctcacacgc caccacgacg tcgagatgac gccggtaaga gggcacctcc atcttggcca
      181 cctccctggc aacatcaact accttcctgt caagccgttc cttgtgcctg gggaacatgg
      241 agttgtacag cagcgaggtg ccacaggata tgctgtaagc gttcaggccc ttctccttga
      301 gccactccag gagctccctc agcgtgatgt tgcccgtcac agtccaccgg tcccagaccg
      361 tccacgacaa ctcctggtgc ttgatggtct tgggtggaac tggttcagca atggagaaga
      421 gagggatagc gaggtttgca aacgtgttgc ggtagtcttc aaccttgtgt ccaccagcaa
      481 gggctttgta aagctcaagg cagacgaggc cagtggccat cgcggtggag gtggcgatag
      541 ctgggatgat cctgccggct ataaacttgg ccttgagctt gtccacttca ggaatgctgt
      601 agttccttgc cctcatgttg gcaaaaccag ctatcacatc catatggaag tttgtgtcat
      661 catccttctc aaactgtatt gggttcatgt ggaaccctga tggcagtgtt ttggaaactt
      721 cttctaactt ggcaataagc tcttcaatga cagcagcgtc gtcaacagat gcagaggaca
      781 gactagtggc cttctcatct gtaactatct taaccccctg ctttggttga aaatccggga
      841 caatcacctt gtcgacagct tcagcagcca gcttgtttgg ggttttggcc cactcaggta
      901 tgggtattcc aaaagtctct gccctcaata ttgcagcagc caaaataaag ct
//
