LOCUS       sequence-164219-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN 
            nuclease 1 inactive mutant)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
            R, Doudna JA
  TITLE     Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA 
            processing and RNA detection.
  JOURNAL   Nature. 2016 Sep 26. doi: 10.1038/nature19802.
  PUBMED    27669025
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 26. doi: 10.1038/nature19802."
COMMENT     Sequence Label: p2CT-His-MBP-Lbu_C2c2_R472A_H477A
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatattactg ggcgtatgcg cggcaaaaca gttaagaaca ataaaggtga agagaagtac
       61 gtgtccggag aagttgataa gatctataat gaaaataaga agaacgaggt taaggagaac
      121 ttaaaaatgt tctattcgta cgatttcaat atggacaaca agaatgaaat cgaagatttc
      181 ttcgccaaca tcgacgaggc gatttcttcc atcgctcacg gtattgtcgc cttcaacttg
      241 gaattagaag gtaaggatat ctttgcgttc aagaacattg cgccatccga aatctcaaag
      301 aagatgtttc agaatgagat taacgagaaa aaactgaaat tgaagatctt tcgtcaactg
      361 aactctgcca acgtgttccg ctatctcgaa aagtataaaa ttctgaatta ccttaaacgt
      421 acacgcttcg agtttgtcaa taaaaatatc ccattcgtcc cgtctttcac caaattatat
      481 tcgcgcattg atgacctgaa gaatagtctt gggatttact ggaaaactcc gaaaacaaac
      541 gacgacaata agactaagga gattattgat gcccaaatct atttgcttaa aaacatctat
      601 tacggggagt tcctgaatta tttcatgtcg aacaatggta atttctttga gatttctaaa
      661 gaaatcatcg aattgaacaa gaacgataaa cgcaacttaa agactgggtt ttacaagctg
      721 caaaagtttg aagacatcca ggagaagatt ccaaaggaat acttggcgaa tatccagtcc
      781 ctgtacatga ttaatgccgg taatcaggac gaagaagaaa aggacactta tattgatttc
      841 attcaaaaga tcttcttaaa gggatttatg acgtatcttg ctaataacgg tcgtttaagt
      901 ctgatttaca tcggctcgga tgaagaaaca aatacgtcat tagcag
//
