LOCUS       sequence-164305-        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid. Expresses EGFP and 
            puromycin resistance in mammalian cells under the control of a 
            doxycycline-inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Panciera T, Azzolin L, Fujimura A, Di Biagio D, Frasson C, Bresolin 
            S, Soligo S, Basso G, Bicciato S, Rosato A, Cordenonsi M, Piccolo S
  TITLE     Induction of Expandable Tissue-Specific Stem/Progenitor Cells 
            through Transient Expression of YAP/TAZ.
  JOURNAL   Cell Stem Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009.
  PUBMED    27641305
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2016 Sep 9. pii: S1934-5909(16)30256-9. doi: 
            10.1016/j.stem.2016.08.009."
COMMENT     Sequence Label: FUW-tetO-EGFP
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(132..731)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(215..235)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     712..731
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(905..924)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga
       61 attgggttcg aaatcgatgc atggggtcgt gcgctccttt cggtcgggcg ctgcgggtcg
      121 tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc
      181 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc
      241 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc
      301 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg
      361 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg
      421 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc
      481 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac
      541 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc
      601 tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg
      661 gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg
      721 tactcggtca tggttgtggc catattatca tcgtgttttt caaaggaaaa ccacgtcccc
      781 gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa agcatgtgca
      841 ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct tcagcccctt
      901 gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac tcacaacgtg
      961 gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt ggccgcagag
     1021 gcacctgtcg ccaggtggg
//
