LOCUS       sequence-164513-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 
            cloning of inserts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: MCV
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..110
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        91..109
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    124..231
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     148..164
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     148..164
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(198..214)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(199..215)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(255..273)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        300..363
                     /gene="bla"
                     /label=AmpR promoter
     CDS             548..926
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(582..601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggccttcg aattaattaa taatacgact cactataggg gacgtccgaa
      121 ttggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa
      181 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac cgtacccagc
      241 ttttgttgca tgccccttta gtgagggtta attttaatta attcgaacga tacttcaaat
      301 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      361 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      421 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      481 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      541 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      601 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      661 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      721 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
      781 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
      841 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
      901 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact ac
//
