LOCUS       sequence-164515-        5184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NOTCH1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NOTCH1_ICN
FEATURES             Location/Qualifiers
     source          1..5184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3063..3162)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3179..3198)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3180..3198)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3203..3219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3203..3219)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3650..4441
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4534..5122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5023..5042
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgcggcggc agcatggcca gctctggttc cctgagggct tcaaagtgtc
      721 tgaggccagc aagaagaagc ggcgggagcc cctcggcgag gactccgtgg gcctcaagcc
      781 cctgaagaac gcttcagacg gtgccctcat ggacgacaac cagaatgagt ggggggacga
      841 ggacctggag accaagaagt tccggttcga ggagcccgtg gttctgcctg acctggacga
      901 ccagacagac caccggcagt ggactcagca gcacctggat gccgctgacc tgcgcatgtc
      961 tgccatggcc cccacaccgc cccagggtga ggttgacgcc gactgcatgg acgtcaatgt
     1021 ccgcgggcct gatggcttca ccccgctcat gatcgcctcc tgcagcgggg gcggcctgga
     1081 gacgggcaac agcgaggaag aggaggacgc gccggccgtc atctccgact tcatctacca
     1141 gggcgccagc ctgcacaacc agacagaccg cacgggcgag accgccttgc acctggccgc
     1201 ccgctactca cgctctgatg ccgccaagcg cctgctggag gccagcgcag atgccaacat
     1261 ccaggacaac atgggccgca ccccgctgca tgcggctgtg tctgccgacg cacaaggtgt
     1321 cttccagatc ctgatccgga accgagccac agacctggat gcccgcatgc atgatggcac
     1381 gacgccactg atcctggctg cccgcctggc cgtggagggc atgctggagg acctcatcaa
     1441 ctcacacgcc gacgtcaacg ccgtagatga cctgggcaag tccgccctgc actgggccgc
     1501 cgccgtgaac aatgtggatg ccgcagttgt gctcctgaag aacggggcta acaaagatat
     1561 gcagaacaac agggaggaga cacccctgtt tctggccgcc cgggagggca gctacgagac
     1621 cgccaaggtg ctgctggacc actttgccaa ccgggacatc acggatcata tggaccgcct
     1681 gccgcgcgac atcgcacagg agcgcatgca tcacgacatc gtgaggctgc tggacgagta
     1741 caacctggtg cgcagcccgc agctgcacgg agccccgctg gggggcacgc ccaccctgtc
     1801 gcccccgctc tgctcgccca acggctacct gggcagcctc aagcccggcg tgcagggcaa
     1861 gaaggtccgc aagcccagca gcaaaggcct ggcctgtgga agcaaggagg ccaaggacct
     1921 caaggcacgg aggaagaagt cccaggacgg caagggctgc ctgctggaca gctccggcat
     1981 gctctcgccc gtggactccc tggagtcacc ccatggctac ctgtcagacg tggcctcgcc
     2041 gccactgctg ccctccccgt tccagcagtc tccgtccgtg cccctcaacc acctgcctgg
     2101 gatgcccgac acccacctgg gcatcgggca cctgaacgtg gcggccaagc ccgagatggc
     2161 ggcgctgggt gggggcggcc ggctggcctt tgagactggc ccacctcgtc tctcccacct
     2221 gcctgtggcc tctggcacca gcaccgtcct gggctccagc agcggagggg ccctgaattt
     2281 cactgtgggc gggtccacca gtttgaatgg tcaatgcgag tggctgtccc ggctgcagag
     2341 cggcatggtg ccgaaccaat acaaccctct gcgggggagt gtggcaccag gccccctgag
     2401 cacacaggcc ccctccctgc agcatggcat ggtaggcccg ctgcacagta gccttgctgc
     2461 cagcgccctg tcccagatga tgagctacca gggcctgccc agcacccggc tggccaccca
     2521 gcctcacctg gtgcagaccc agcaggtgca gccacaaaac ttacagatgc agcagcagaa
     2581 cctgcagcca gcaaacatcc agcagcagca aagcctgcag ccgccaccac caccaccaca
     2641 gccgcacctt ggcgtgagct cagcagccag cggccacctg ggccggagct tcctgagtgg
     2701 agagccgagc caggcagacg tgcagccact gggccccagc agcctggcgg tgcacactat
     2761 tctgccccag gagagccccg ccctgcccac gtcgctgcca tcctcgctgg tcccacccgt
     2821 gaccgcagcc cagttcctga cgcccccctc gcagcacagc tactcctcgc ctgtggacaa
     2881 cacccccagc caccagctac aggtgcctga gcaccccttc ctcaccccgt cccctgagtc
     2941 ccctgaccag tggtccagct cgtccccgca ttccaacgtc tccgactggt ccgagggcgt
     3001 ctccagccct cccaccagca tgcagtccca gatcgcccgc atcccggagg cgttcaagta
     3061 attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3121 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3181 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3241 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     3301 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     3361 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     3421 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     3481 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     3541 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     3601 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     3661 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     3721 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     3781 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     3841 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     3901 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     3961 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     4021 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     4081 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     4141 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     4201 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     4261 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     4321 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     4381 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     4441 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     4501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4681 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5101 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5161 ttgctggcct tttgctcaca tgtt
//
