LOCUS       sequence-164545-         406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes the synthetic coiled coil peptide SYNZIP13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Thompson KE, Bashor CJ, Lim WA, Keating AE
  TITLE     SYNZIP protein interaction toolbox: in vitro and in vivo 
            specifications of heterospecific coiled-coil interaction domains.
  JOURNAL   ACS Synth Biol. 2012 Apr 20;1(4):118-29.
  PUBMED    22558529
REFERENCE   2  (bases 1 to 406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol."; date: "2012-04-20"; volume: "1(4)"; pages: "118-29"
COMMENT     Sequence Label: pENTR-SYNZIP13
FEATURES             Location/Qualifiers
     source          1..406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..80
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(264..363)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(380..399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(381..399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac
       61 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgggatccc agaaagttga
      121 agaactgaaa aacaaaatcg cggaactgga aaaccgtaac gcggttaaaa agaaccgtgt
      181 tgcgcacctg aaacaggaaa tcgcttatct gaaagacgaa ctggcggctc acgaatttga
      241 atgataaaag ggtgggcgcg ccgacccagc tttcttgtac aaagttggca ttataagaaa
      301 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
      361 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtc
//
