LOCUS       sequence-164590-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) 
            labeling of endogenous proteins with a TRP selection marker, 
            originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-g
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    926..959
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     960..979
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        960..978
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgagtgtca taaactttac cggatcgagt ggacctctcg tcaaggtatg cgggctccag
       61 tccactgaag cagcggagtg tgccctagac tctgatgccg atctcttggg cattatctgc
      121 gtcccgaata ggaaacgcac cattgatcct gtaatcgcgc gcaagatttc ttcgctcgta
      181 aaggcctaca agaactcttc aggaacgccg aagtatttag tcggtgtttt tcgtaatcag
      241 cctaaagagg acgtattggc actcgtaaac gactacggca tagatatagt gcagttacat
      301 ggggacgaga gctggcagga atatcaagag ttcctgggct tgcctgtcat taaacgactg
      361 gtttttccga aagattgtaa tatccttttg tcagcagcgt cgcagaaacc gcactcattc
      421 ataccgctct tcgactcgga agcaggcggt acaggggaac tactggactg gaatagtata
      481 agtgactggg tcggacggca agagagtccg gagtccttac attttatgct ggccgggggt
      541 ttaacccccg aaaacgtggg agatgccctc cgattaaatg gggttatcgg tgtcgatgta
      601 tcaggtgggg tggagacgaa tggcgtgaag gactcaaata aaatcgctaa ttttgtgaaa
      661 aacgctaaga aatgagaata ttgataaatt taaattttgg aatgagggtt gtcctttcct
      721 ttacaataga ggcatttgag tgagtaggtt ttatcacctt cttgctctta ttaaatcgca
      781 gttgtctttc cggtatgtgc ttgactgagg aacaatattt tattcatgat acatttccat
      841 gctacgcata tcgtcatcca catggaaggt aattttcttc tgtggccttt catatagttg
      901 tgaatggtag aacaatcttc atataataac ttcgtataat gtatgctata cgaagttatt
      961 aatacgactc actatagggc gaattggagc tccaattcgg acgtccccta tagtg
//
