LOCUS       sequence-164596-        6816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ERBB3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ERBB3_WT
FEATURES             Location/Qualifiers
     source          1..6816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4695..4794)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4811..4830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4812..4830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4835..4851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4835..4851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5282..6073
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6166..6754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6655..6674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgagggcga acgacgctct gcaggtgctg ggcttgcttt tcagcctggc
      721 ccggggctcc gaggtgggca actctcaggc agtgtgtcct gggactctga atggcctgag
      781 tgtgaccggc gatgctgaga accaatacca gacactgtac aagctctacg agaggtgtga
      841 ggtggtgatg gggaaccttg agattgtgct cacgggacac aatgccgacc tctccttcct
      901 gcagtggatt cgagaagtga caggctatgt cctcgtggcc atgaatgaat tctctactct
      961 accattgccc aacctccgcg tggtgcgagg gacccaggtc tacgatggga agtttgccat
     1021 cttcgtcatg ttgaactata acaccaactc cagccacgct ctgcgccagc tccgcttgac
     1081 tcagctcacc gagattctgt cagggggtgt ttatattgag aagaacgata agctttgtca
     1141 catggacaca attgactgga gggacatcgt gagggaccga gatgctgaga tagtggtgaa
     1201 ggacaatggc agaagctgtc ccccctgtca tgaggtttgc aaggggcgat gctggggtcc
     1261 tggatcagaa gactgccaga cattgaccaa gaccatctgt gctcctcagt gtaatggtca
     1321 ctgctttggg cccaacccca accagtgctg ccatgatgag tgtgccgggg gctgctcagg
     1381 ccctcaggac acagactgct ttgcctgccg gcacttcaat gacagtggag cctgtgtacc
     1441 tcgctgtcca cagcctcttg tctacaacaa gctaactttc cagctggaac ccaatcccca
     1501 caccaagtat cagtatggag gagtttgtgt agccagctgt ccccataact ttgtggtgga
     1561 tcaaacatcc tgtgtcaggg cctgtcctcc tgacaagatg gaagtagata aaaatgggct
     1621 caagatgtgt gagccttgtg ggggactatg tcccaaagcc tgtgagggaa caggctctgg
     1681 gagccgcttc cagactgtgg actcgagcaa cattgatgga tttgtgaact gcaccaagat
     1741 cctgggcaac ctggactttc tgatcaccgg cctcaatgga gacccctggc acaagatccc
     1801 tgccctggac ccagagaagc tcaatgtctt ccggacagta cgggagatca caggttacct
     1861 gaacatccag tcctggccgc cccacatgca caacttcagt gttttttcca atttgacaac
     1921 cattggaggc agaagcctct acaaccgggg cttctcattg ttgatcatga agaacttgaa
     1981 tgtcacatct ctgggcttcc gatccctgaa ggaaattagt gctgggcgta tctatataag
     2041 tgccaatagg cagctctgct accaccactc tttgaactgg accaaggtgc ttcgggggcc
     2101 tacggaagag cgactagaca tcaagcataa tcggccgcgc agagactgcg tggcagaggg
     2161 caaagtgtgt gacccactgt gctcctctgg gggatgctgg ggcccaggcc ctggtcagtg
     2221 cttgtcctgt cgaaattata gccgaggagg tgtctgtgtg acccactgca actttctgaa
     2281 tggggagcct cgagaatttg cccatgaggc cgaatgcttc tcctgccacc cggaatgcca
     2341 acccatggag ggcactgcca catgcaatgg ctcgggctct gatacttgtg ctcaatgtgc
     2401 ccattttcga gatgggcccc actgtgtgag cagctgcccc catggagtcc taggtgccaa
     2461 gggcccaatc tacaagtacc cagatgttca gaatgaatgt cggccctgcc atgagaactg
     2521 cacccagggg tgtaaaggac cagagcttca agactgttta ggacaaacac tggtgctgat
     2581 cggcaaaacc catctgacaa tggctttgac agtgatagca ggattggtag tgattttcat
     2641 gatgctgggc ggcacttttc tctactggcg tgggcgccgg attcagaata aaagggctat
     2701 gaggcgatac ttggaacggg gtgagagcat agagcctctg gaccccagtg agaaggctaa
     2761 caaagtcttg gccagaatct tcaaagagac agagctaagg aagcttaaag tgcttggctc
     2821 gggtgtcttt ggaactgtgc acaaaggagt gtggatccct gagggtgaat caatcaagat
     2881 tccagtctgc attaaagtca ttgaggacaa gagtggacgg cagagttttc aagctgtgac
     2941 agatcatatg ctggccattg gcagcctgga ccatgcccac attgtaaggc tgctgggact
     3001 atgcccaggg tcatctctgc agcttgtcac tcaatatttg cctctgggtt ctctgctgga
     3061 tcatgtgaga caacaccggg gggcactggg gccacagctg ctgctcaact ggggagtaca
     3121 aattgccaag ggaatgtact accttgagga acatggtatg gtgcatagaa acctggctgc
     3181 ccgaaacgtg ctactcaagt cacccagtca ggttcaggtg gcagattttg gtgtggctga
     3241 cctgctgcct cctgatgata agcagctgct atacagtgag gccaagactc caattaagtg
     3301 gatggccctt gagagtatcc actttgggaa atacacacac cagagtgatg tctggagcta
     3361 tggtgtgaca gtttgggagt tgatgacctt cggggcagag ccctatgcag ggctacgatt
     3421 ggctgaagta ccagacctgc tagagaaggg ggagcggttg gcacagcccc agatctgcac
     3481 aattgatgtc tacatggtga tggtcaagtg ttggatgatt gatgagaaca ttcgcccaac
     3541 ctttaaagaa ctagccaatg agttcaccag gatggcccga gacccaccac ggtatctggt
     3601 cataaagaga gagagtgggc ctggaatagc ccctgggcca gagccccatg gtctgacaaa
     3661 caagaagcta gaggaagtag agctggagcc agaactagac ctagacctag acttggaagc
     3721 agaggaggac aacctggcaa ccaccacact gggctccgcc ctcagcctac cagttggaac
     3781 acttaatcgg ccacgtggga gccagagcct tttaagtcca tcatctggat acatgcccat
     3841 gaaccagggt aatcttgggg agtcttgcca ggagtctgca gtttctggga gcagtgaacg
     3901 gtgcccccgt ccagtctctc tacacccaat gccacgggga tgcctggcat cagagtcatc
     3961 agaggggcat gtaacaggct ctgaggctga gctccaggag aaagtgtcaa tgtgtaggag
     4021 ccggagcagg agccggagcc cacggccacg cggagatagc gcctaccatt cccagcgcca
     4081 cagtctgctg actcctgtta ccccactctc cccacccggg ttagaggaag aggatgtcaa
     4141 cggttatgtc atgccagata cacacctcaa aggtactccc tcctcccggg aaggcaccct
     4201 ttcttcagtg ggtctcagtt ctgtcctggg tactgaagaa gaagatgaag atgaggagta
     4261 tgaatacatg aaccggagga gaaggcacag tccacctcat ccccctaggc caagttccct
     4321 tgaggagctg ggttatgagt acatggatgt ggggtcagac ctcagtgcct ctctgggcag
     4381 cacacagagt tgcccactcc accctgtacc catcatgccc actgcaggca caactccaga
     4441 tgaagactat gaatatatga atcggcaacg agatggaggt ggtcctgggg gtgattatgc
     4501 agccatgggg gcctgcccag catctgagca agggtatgaa gagatgagag cttttcaggg
     4561 gcctggacat caggcccccc atgtccatta tgcccgccta aaaactctac gtagcttaga
     4621 ggctacagac tctgcctttg ataaccctga ttactggcat agcaggcttt tccccaaggc
     4681 taatgcccag agaacgccaa ctttcttgta caaagttggc attataagaa agcattgctt
     4741 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     4801 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     4861 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
     4921 cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
     4981 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
     5041 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     5101 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
     5161 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     5221 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     5281 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     5341 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     5401 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     5461 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     5521 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     5581 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     5641 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     5701 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     5761 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     5821 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     5881 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     5941 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     6001 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     6061 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6781 gttcctggcc ttttgctggc cttttgctca catgtt
//
