LOCUS       sequence-164603-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector TRP/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a TRP selection 
            marker, originating from pSIVt (ID:81092).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTT-ir
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    915..948
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     949..968
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        949..967
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaactttacc ggatcgagtg gacctctcgt caaggtatgc gggctccagt ccactgaagc
       61 agcggagtgt gccctagact ctgatgccga tctcttgggc attatctgcg tcccgaatag
      121 gaaacgcacc attgatcctg taatcgcgcg caagatttct tcgctcgtaa aggcctacaa
      181 gaactcttca ggaacgccga agtatttagt cggtgttttt cgtaatcagc ctaaagagga
      241 cgtattggca ctcgtaaacg actacggcat agatatagtg cagttacatg gggacgagag
      301 ctggcaggaa tatcaagagt tcctgggctt gcctgtcatt aaacgactgg tttttccgaa
      361 agattgtaat atccttttgt cagcagcgtc gcagaaaccg cactcattca taccgctctt
      421 cgactcggaa gcaggcggta caggggaact actggactgg aatagtataa gtgactgggt
      481 cggacggcaa gagagtccgg agtccttaca ttttatgctg gccgggggtt taacccccga
      541 aaacgtggga gatgccctcc gattaaatgg ggttatcggt gtcgatgtat caggtggggt
      601 ggagacgaat ggcgtgaagg actcaaataa aatcgctaat tttgtgaaaa acgctaagaa
      661 atgagaatat tgataaattt aaattttgga atgagggttg tcctttcctt tacaatagag
      721 gcatttgagt gagtaggttt tatcaccttc ttgctcttat taaatcgcag ttgtctttcc
      781 ggtatgtgct tgactgagga acaatatttt attcatgata catttccatg ctacgcatat
      841 cgtcatccac atggaaggta attttcttct gtggcctttc atatagttgt gaatggtaga
      901 acaatcttca tataataact tcgtataatg tatgctatac gaagttatta atacgactca
      961 ctatagggcg aattgga
//
