LOCUS       sequence-164757-         541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for secretion of N-terminal OsmY, deca-histidine, SUMO tagged 
            gEEHE_02.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Bhardwaj G, Mulligan VK, Bahl CD, Gilmore JM, Harvey PJ, Cheneval O,
            Buchko GW, Pulavarti SV, Kaas Q, Eletsky A, Huang PS, Johnsen WA, 
            Greisen PJ, Rocklin GJ, Song Y, Linsky TW, Watkins A, Rettie SA, Xu 
            X, Carter LP, Bonneau R, Olson JM, Coutsias E, Correnti CE, 
            Szyperski T, Craik DJ, Baker D
  TITLE     Accurate de novo design of hyperstable constrained peptides.
  JOURNAL   Nature. 2016 Sep 14. doi: 10.1038/nature19791.
  PUBMED    27626386
REFERENCE   2  (bases 1 to 541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Sep 14. doi: 10.1038/nature19791."
COMMENT     Sequence Label: pCDB289
FEATURES             Location/Qualifiers
     source          1..541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(445..474)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatcctca ttaaccgcag tgaacacggc agttggtaac
       61 acccagttta cggcacagac gctcacattc ttcagaagaa gaaacggtgt aggtttcacc
      121 gttcacgtcg cattcgcacg gagcgccgcc aatctgttca cggtgggctt caatgatgtc
      181 gttgtcttcc atgtccaggt cttccggcgc ttgatctgcc tggatgcgaa taccgtcata
      241 caggaaacgc aggctatcca tttccttgcc ctgacgtttg gcaaacgctt ccatcaggcg
      301 acgcagcggc gtggtctttt tgatcttaaa gaaaatttca gagctgccgt cactaacttt
      361 caggttaata tgggtttccg gtttcacttc cggcttgact tccggctttg cttcttggtt
      421 gacttccgaa tcttgcaggc taccgtggtg gtggtggtgg tggtggtggt ggtgacccat
      481 cttagttttc agatcatttt taacgctttt cacaccatct accgctttgg cgatactttc
      541 a
//
