LOCUS       sequence-164794-        1071 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST-CD (Cbx7) fusion proteins in E. coli. Cbx7 
            chromodomain only; amino acids 1–62..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1071)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-CD(Cbx7)
FEATURES             Location/Qualifiers
     source          1..1071
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(307..329)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(474..492)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        560..664
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(883..902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatcggatc tggaagttct gttccagggg cccctgggat ccccggaatt catggagctg
       61 tcagccatcg gcgagcaggt gttcgccgtg gagagcatcc ggaagaagcg cgtgcggaag
      121 ggtaaagtcg agtatctggt gaagtggaaa ggatggcccc caaagtacag cacgtgggag
      181 ccagaagagc acatcttgga cccccgcctc gtcatggcct acgaggagaa ggaggagtga
      241 gctcgagcgg ccgcatcgtg actgactgac gatctgcctc gcgcgtttcg gtgatgacgg
      301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
      361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
      421 catgacccag tcacgtagcg atagcggagt gtataattct tgaagacgaa agggcctcgt
      481 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      901 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac t
//
