LOCUS       sequence-164819-         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway-compatible tdTomato fusion reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne 
            P
  TITLE     A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate 
            Expression Constructs with Diverse Research Applications.
  JOURNAL   PLoS One. 2016 Aug 8;11(8):e0159277. doi: 
            10.1371/journal.pone.0159277. eCollection 2016.
  PUBMED    27500400
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-8"; volume: "11(8):e0159277. doi"; pages: " 
            10.1371/journal.pone.0159277. eCollection 2016"
COMMENT     Sequence Label: pME-tdTomato no stop
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(241..259)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     509..528
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     703..726
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggctccgc cgccaccatg gtgagcaagg
      121 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
      181 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      241 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
      301 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
      361 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
      421 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
      481 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
      541 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
      601 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
      661 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
      721 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
      781 tcctgg
//
