LOCUS       sequence-164820-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutant GST-CD-ATLm (Cbx7) fusion proteins in E. coli. Cbx7
            amino acids 1-84 with mutation of RKR70-72, K75, and KR77-78 to 
            AGA70-72, A75, and AG77-78..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     pTRIPZ-MSC1-Halotag-Cbx2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGEX-6P-1-GST-CD-ATLm(Cbx7)
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..33
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(373..395)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(540..558)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        626..730
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(949..968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaatcggatc tggaagttct gttccagggg cccctgggat ccccggaatt catggagctg
       61 tcagccatcg gcgagcaggt gttcgccgtg gagagcatcc ggaagaagcg cgtgcggaag
      121 ggtaaagtcg agtatctggt gaagtggaaa ggatggcccc caaagtacag cacgtgggag
      181 ccagaagagc acatcttgga cccccgcctc gtcatggcct acgaggagaa ggaggagaga
      241 gaccgagcat cggggtatgc aggcgcaggt ccggcacccg caggccttct gctgcagcgg
      301 ctgtgagctc gagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga
      361 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      421 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      481 cgcagccatg acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg
      541 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      601 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      661 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      721 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      781 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      841 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      901 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      961 ggtattatcc cgtgttgacg ccgggcaaga gcaac
//
