LOCUS       sequence-164826-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV backbone with a minimal H1/TO promoter driving expression of a 
            sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with 
            SpCas9. gRNA transcription is doxycycline dependent..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   de Solis CA, Ho A, Holehonnur R, Ploski JE
  TITLE     The Development of a Viral Mediated CRISPR/Cas9 System with 
            Doxycycline Dependent gRNA Expression for Inducible In vitro and In 
            vivo Genome Editing.
  JOURNAL   Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070.
            eCollection 2016.
  PUBMED    27587996
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2016-08-18"; volume: "9:70. doi"; pages: " 
            10.3389/fnmol.2016.00070. eCollection 2016"
COMMENT     Sequence Label: 
            pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(372..393)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     721..740
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tcctaggtgg gaggtggccc gttggtgtac cgcagcatgg ggtagaagga tcgggcaaag
      121 ttgttggcct gggtgcagct gtagtcatct tcagaggcag gtagcaggca ggccaggagc
      181 agcagcagca gcagaagcag ctgcaacggt agcgctgccc tgatcaccct tgagaggaag
      241 gagcgtctcg gctgggagct gccggggctg tcgaggtggg gcatcctgct gtctgtctcc
      301 tcctcctcct ctcgagatct gagtccggac ttgtacagct cgtccatgcc gagagtgatc
      361 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      421 ctcagggcgg actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      481 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      541 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      601 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      661 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      721 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc
      781 atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc
      841 acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg
      901 cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg
      961 ctgaacttgt ggccgtttac gtcgccgtcc agctcgac
//
