LOCUS       sequence-164831-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  receives miRNA target sequences for testing using the dual 
            luciferase assay system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Moyle RL, Carvalhais LC, Pretorius LS, Nowak E, Subramaniam G, 
            Dalton-Morgan J, Schenk PM
  TITLE     An Optimized Transient Dual Luciferase Assay for Quantifying 
            MicroRNA Directed Repression of Targeted Sequences.
  JOURNAL   Front Plant Sci. 2017 Sep 20;8:1631. doi: 10.3389/fpls.2017.01631. 
            eCollection 2017.
  PUBMED    28979287
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2017-09-20"; volume: "8:1631. doi"; pages: " 
            10.3389/fpls.2017.01631. eCollection 2017"
COMMENT     Sequence Label: pGrDL_SP
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..938)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 gattattgtt catttttgag aactcgctca acgaacgatt tgatatattt tcccatttca
       61 tcaggtgcat cttcttgcga aaaatgaaga ccttttactt tgacaaattc agtattagga
      121 aacttcttgg caccttcaac aatagcattg gaaaagaatc ctgggtccga ttcaataaac
      181 atttttggta aatcatcact tgcacgtaga taagcattat aattcctaac aatttgtaca
      241 acgtcaggtt taccaccttt tactaacggg atttcacgag gccatgataa tgttggacga
      301 cgaacttcac ctttctcttt gaatggttca agatatgctg caaattcttc tggttctaac
      361 tttctcatga tttttgatgg caacatggtt tccacgaaga agttattctc caaaaccatt
      421 ttttctcctt cttcagattt gatcaacgca atatcttctt caatatcagg ccattcatcc
      481 catgattcaa tcacatctac tacactttca gcgtgaacta ttgctttgat cttatcttga
      541 tgctcatagc tataatgaaa tgccaaacaa gcaccccaat catggccgac aaaaatgatc
      601 ttctttggta aattaagaag ttcaaaccat gcagtaagat atttgtaatg atcaagtaac
      661 ctataagaac cattaccaga tttgcctgat ttgcccatac caataaggtc tggtataata
      721 caccgcgcta ctggctcaat atgtggcaca acatgtcgcc ataaataaga agaggccgcg
      781 ttaccatgta aaaaaataac agcattttct gcatgttttt ctgaatcata ataattaata
      841 aatgaatcaa gaacattcat ttgtttacat ctggcccacc actgcggacc agttatcatc
      901 cgtttccttt gttctggatc ataaactttc gaagtcatgt cgatcgacct cgagttgaga
      961 gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt
     1021 tt
//
