LOCUS       sequence-164861-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses m23 peptidase from Campylobacter jejuni.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  TITLE     C.jejuni_peptidase m23 family
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET21a(+)_C.jejuni m23 peptidase
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             814..831
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(884..902)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      898..945
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgga actcatcaaa
       61 ggtcaagctt tatttttgga actagataag aaagattttt tatctttgaa aaataatgat
      121 aaaaatattc ccacctttgc acatcctaaa aatcaagaaa aaattctagc tattttttcc
      181 ttaccttaca aaaatccccc gcaaaataca aaacttatcg cattttataa agataaaaaa
      241 gaagaaattt tcataaaaac tttagaaggt aattataaaa gtgaaaaatt gcaagttgaa
      301 aacaaaaaga tttttccacc aaaaaccata caagaacgca tcgctaaaga attaaaagaa
      361 gccaatgcga tttatagttc ttatactcca aaagctttat ttaacggtgc ttttaatata
      421 cctttaaatt cttttatcac aagtgatttt ggcaaagcaa gaacttttaa tgaaaaagta
      481 gcaagttatc atagtggaac ggactttaga gccgccacag gaacgcctat ttatgcagcc
      541 aattcaggtg tagtaaaaat tgcaaaagat cgttatttcg caggaaattc agtagtcatt
      601 gatcatggtt ttggaattta ttcacaatat tatcatcttt ctaaaatcga tgttaaagta
      661 ggacaaaaga taaaaaaagg tgaacttata ggacttagtg gggctagtgg tagggtaagt
      721 gggccgcatt tgcattttgg aattttagct ggaggcaaac aagttgatcc tttggatttt
      781 gtatccaaat ttaatgctat ttttcaactc gagcaccacc accaccacca ctgagatccg
      841 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      901 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      961 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gc
//
