LOCUS       sequence-164943-        4062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing MAPK9 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_MAPK9_WT
FEATURES             Location/Qualifiers
     source          1..4062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1462..1561)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1578..1597)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1579..1597)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1602..1618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1602..1618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2049..2840
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2933..3521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3422..3441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3675..3692
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3851..3878)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3970..4056)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gagcgacagt aaatgtgaca gtcagtttta tagtgtgcaa gtggcagact
      241 caaccttcac tgtcctaaaa cgttaccagc agctgaaacc aattggctct ggggcccaag
      301 ggattgtttg tgctgcattt gatacagttc ttgggataaa tgttgcagtc aagaaactaa
      361 gccgtccttt tcagaaccaa actcatgcaa agagagctta tcgtgaactt gtcctcttaa
      421 aatgtgtcaa tcataaaaat ataattagtt tgttaaatgt gtttacacca caaaaaactc
      481 tagaagaatt tcaagatgtg tatttggtta tggaattaat ggatgctaac ttatgtcagg
      541 ttattcacat ggagctggat catgaaagaa tgtcctacct tctttaccag atgctttgtg
      601 gtattaaaca tctgcattca gctggtataa ttcatagaga tttgaagcct agcaacattg
      661 ttgtgaaatc agactgcacc ctgaagatcc ttgactttgg cctggcccgg acagcgtgca
      721 ctaacttcat gatgacccct tacgtggtga cacggtacta ccgggcgccc gaagtcatcc
      781 tgggtatggg ctacaaagag aacgttgata tctggtcagt gggttgcatc atgggagagc
      841 tggtgaaagg ttgtgtgata ttccaaggca ctgaccatat tgatcagtgg aataaagtta
      901 ttgagcagct gggaacacca tcagcagagt tcatgaagaa acttcagcca actgtgagga
      961 attatgtcga aaacagacca aagtatcctg gaatcaaatt tgaagaactc tttccagatt
     1021 ggatattccc atcagaatct gagcgagaca aaataaaaac aagtcaagcc agagatctgt
     1081 tatcaaaaat gttagtgatt gatcctgaca agcggatctc tgtagacgaa gctctgcgtc
     1141 acccatacat cactgtttgg tatgaccccg ccgaagcaga agccccacca cctcaaattt
     1201 atgatgccca gttggaagaa agagaacatg caattgaaga atggaaagag ctaatttaca
     1261 aagaagtcat ggattgggaa gaaagaagca agaatggtgt tgtaaaagat cagccttcag
     1321 atgcagcagt aagtagcaac gccactcctt ctcagtcttc atcgatcaat gacatttcat
     1381 ccatgtccac tgagcagacg ctggcctcag acacagacag cagtcttgat gcctcgacgg
     1441 gaccccttga aggctgtcga tgcccaactt tcttgtacaa agttggcatt ataagaaagc
     1501 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1561 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1621 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1681 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     1741 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     1801 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     1861 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     1921 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     1981 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2041 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2101 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2161 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2221 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2281 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2341 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2401 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2461 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2521 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2581 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2641 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2701 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     2761 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     2821 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     2881 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3061 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3601 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3661 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3721 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3781 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     3841 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     3901 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     3961 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4021 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
